缺血對心肌中連結蛋白43和乙酰膽堿M受體亞型M3(M3mAChR)結合的影響課件

1、缺血對心肌中連結蛋白43和乙酰膽堿M受體亞型M3(M3mAChR)結合的影響Ischemia Impairs the Association Between Connexin 43 and M3 Subtype of Acetylcholine Muscarinic Receptor (M3-mAChR) in Ventricle MyocytesDe-Li Dong Department of Pharmacology, Harbin Medical University, Harbin, P.R.China缺血對心肌中連結蛋白43和乙酰膽堿M受體亞型M3(M3mAChR)結合的影響Intr

2、oductionMultiple subtypes of muscarinic acetylcholine receptors expressed in myocardium, including M1,M2,M3,M5-R. (Mol Pharmacol. 2001 ;59:1029-36.) The M3 Receptor-mediated K+ Current (IKM3), a Gq Protein-coupled K+ Channel. (J Biol Chem.2004, 279, 21774-8) Connexin 43(Cx43)-gap junction channels.

3、(Cell 1996;84(3):381-8.)Vagal nerve stimulation exerts antiarrhythmogenic effects accompanied by prevention of the loss of Cx43 during acute MI.(Circulation. 2005 ;112(2):164-70.)缺血對心肌中連結蛋白43和乙酰膽堿M受體亞型M3(M3mAChR)結合的影響The association between Connexin43 and M3-mAChR in ventricular myocytes.The role of

4、 M3-mAChR in cytoprotection of myocardial infarction.Introduction缺血對心肌中連結蛋白43和乙酰膽堿M受體亞型M3(M3mAChR)結合的影響 Results1.Expression of M3-R and Connexin 43 in the heart.缺血對心肌中連結蛋白43和乙酰膽堿M受體亞型M3(M3mAChR)結合的影響A confocal image showing the expression of connexin 43 in myocardium under control conditions and aft

5、er ischemia.缺血對心肌中連結蛋白43和乙酰膽堿M受體亞型M3(M3mAChR)結合的影響A confocal image showing the expression of connexin 43 (red) and M3-mAChR in a isolated myocyte.缺血對心肌中連結蛋白43和乙酰膽堿M受體亞型M3(M3mAChR)結合的影響2. Connexin 43 is coimmunoprecipitated with M2/M3-R缺血對心肌中連結蛋白43和乙酰膽堿M受體亞型M3(M3mAChR)結合的影響3. The effects of ischemia

6、on the expression of M-R and connexin 43. 缺血對心肌中連結蛋白43和乙酰膽堿M受體亞型M3(M3mAChR)結合的影響The effect of ischemia on the expression of connexin 43 in the plasma membrane. 缺血對心肌中連結蛋白43和乙酰膽堿M受體亞型M3(M3mAChR)結合的影響The effect of ischemia on the association between M2 or M3-mAChR and connexin 43. 缺血對心肌中連結蛋白43和乙酰膽堿M受體

7、亞型M3(M3mAChR)結合的影響4. The effect of choline pretreatment on the association between M3/M2 mAChR and connexin 43. 缺血對心肌中連結蛋白43和乙酰膽堿M受體亞型M3(M3mAChR)結合的影響5. Effects of Choline on Hemodynamic Parameters during Coronary Artery Occlusion.缺血對心肌中連結蛋白43和乙酰膽堿M受體亞型M3(M3mAChR)結合的影響6. Effects of choline on ischem

8、ic arrhythmias and the potential role of M3-mAChRs. 缺血對心肌中連結蛋白43和乙酰膽堿M受體亞型M3(M3mAChR)結合的影響7. Effect of choline on infarct size and the potential role of M3-mAChRs. 缺血對心肌中連結蛋白43和乙酰膽堿M受體亞型M3(M3mAChR)結合的影響8. Effect of choline on cardiomyocytes apoptosis and the potential role of M3-mAChRs. 缺血對心肌中連結蛋白43和乙酰膽堿M受體亞型M3(M3mAChR)結合的影響ConclusionsvConnexin 43 interacts with M2/M3-mAChRvIschemia specifically impairs the association between M3-mAChR and connexin 43.vCholine improves