的生物信息學(xué)分析

1、ZNF652的生物信息學(xué)分析 2006級(jí)本碩3班 劉文藝 指導(dǎo)老師：吳炳禮，許麗艷，李恩民該基因官方符號(hào)是ZNF652，名稱是鋅指蛋白652，其它名稱有DKFZp781E2122, KIAA0924。其染色體定位是：17q21.32。一預(yù)測信號(hào)肽及切割位點(diǎn)序列預(yù)測結(jié)果顯示沒有信號(hào)肽序列。二預(yù)測核定位信號(hào)序列MSHTASSCQELVENCAVHVAGMAQEDSRRGQVPSSFYHGANQELDLSTKVYKRESGSPYSVLVDTKMSKPHLHETEEQPYFRETRAVSDVHAVKEDRENSDDTEEEEEEVSYKREQIIVEVNLNNQTLNVSKGEKGVSSQSKETPV

2、LKTSSEEEEEESEEEATDDSNDYGENEKQKKKEKIVEKVSVTQRRTRRAASVAAATTSPTPRTTRGRRKSVEPPKRKKRATKEPKAPVQKAKCEEKETLTCEKCPRVFNTRWYLEKHMNVTHRRMQICDKCGKKFVLESELSLHQQTDCEKNIQCVSCNKSFKKLWSLHEHIKIVHGYAEKKFSCEICEKKFYTMAHVRKHMVAHTKDMPFTCETCGKSFKRSMSLKVHSLQHSGEKPFRCENCDERFQYKYQLRSHMSIHIGHKQFMCQWCGKDFNMKQYFDEHMKTHTGEKPFICEIC

3、GKSFTSRPNMKRHRRTHTGEKPYPCDVCGQRFRFSNMLKAHKEKCFRVTSPVNVPPAVQIPLTTSPATPVPSVVNTATTPTPPINMNPVSTLPPRPIPHPFSHLHIHPHPHHPHHLPIPPVPHLPPPPALFKSEPLNHRGQSEDNFLRHLAEKNSSAQHH核定位信號(hào)位于基因序列中的紅色部分。三蛋白質(zhì)的質(zhì)量和等電點(diǎn)的計(jì)算# PhosphatesMolecular WeightIsoelectric Point069799.88888.92169877.85288.84269955.81688.75370033.78088.65470

4、111.74488.54570189.70888.41670267.67288.25770345.63688.07870423.60087.87這里的分子質(zhì)量的計(jì)算是源自1997IUPAC（國際理論和應(yīng)用化學(xué)聯(lián)合會(huì)）的標(biāo)準(zhǔn)原子質(zhì)量法則。結(jié)果如上表所示。四N型糖基化修飾預(yù)測MSHTASSCQELVENCAVHVAGMAQEDSRRGQVPSSFYHGANQELDLSTKVYKRESGSPYSVLVDTKMSKPHLHETEEQPY 80 FRETRAVSDVHAVKEDRENSDDTEEEEEEVSYKREQIIVEVNLNNQTLNVSKGEKGVSSQSKETPVLKTSSEEEEEESEE

5、 160 EATDDSNDYGENEKQKKKEKIVEKVSVTQRRTRRAASVAAATTSPTPRTTRGRRKSVEPPKRKKRATKEPKAPVQKAKCE 240 EKETLTCEKCPRVFNTRWYLEKHMNVTHRRMQICDKCGKKFVLESELSLHQQTDCEKNIQCVSCNKSFKKLWSLHEHIKI 320 VHGYAEKKFSCEICEKKFYTMAHVRKHMVAHTKDMPFTCETCGKSFKRSMSLKVHSLQHSGEKPFRCENCDERFQYKYQL 400 RSHMSIHIGHKQFMCQWCGKDFNMKQYFDEHMKTHTGEKP

6、FICEICGKSFTSRPNMKRHRRTHTGEKPYPCDVCGQRFRF 480 SNMLKAHKEKCFRVTSPVNVPPAVQIPLTTSPATPVPSVVNTATTPTPPINMNPVSTLPPRPIPHPFSHLHIHPHPHHPH 560 HLPIPPVPHLPPPPALFKSEPLNHRGQSEDNFLRHLAEKNSSAQHH (Threshold=0.5)-SeqName Position Potential Jury N-Glyc agreement result-Sequence 125 NQTL 0.5627 (7/9) + Sequence 129 NVSK

7、0.7168 (9/9) + Sequence 265 NVTH 0.7480 (9/9) + Sequence 305 NKSF 0.3660 (9/9) - Sequence 600 NSSA 0.3511 (7/9) - -結(jié)果發(fā)現(xiàn)有多處N型糖基化修飾，Asn-Xaa-Ser/Thr 和 Asn-Pro-Ser/Thr 在上面序列里用藍(lán)色表示，其中進(jìn)行糖基化的天冬酰胺 用紅色標(biāo)色。五O型糖基化修飾分析 Name S/T Pos G-score I-score Y/N Comment-Sequence S 2 0.376 0.048 . -Sequence T 4 0.421 0.029

8、. -Sequence S 6 0.289 0.023 . -Sequence S 7 0.262 0.017 . -Sequence S 27 0.160 0.022 . -Sequence S 34 0.173 0.033 . -Sequence S 35 0.177 0.020 . -Sequence S 47 0.207 0.060 . -Sequence T 48 0.298 0.037 . -Sequence S 55 0.184 0.031 . -Sequence S 57 0.185 0.021 . -Sequence S 60 0.216 0.061 . -Sequence

9、T 65 0.342 0.036 . -Sequence S 68 0.239 0.193 . -Sequence T 75 0.346 0.045 . -Sequence T 84 0.265 0.032 . -Sequence S 88 0.221 0.046 . -Sequence S 100 0.228 0.053 . -Sequence T 103 0.317 0.030 . -Sequence S 111 0.129 0.065 . -Sequence T 127 0.163 0.039 . -Sequence S 131 0.189 0.056 . -Sequence S 138

10、 0.239 0.057 . -Sequence S 139 0.258 0.047 . -Sequence S 141 0.320 0.052 . -Sequence T 144 0.482 0.053 . -Sequence T 149 0.529 0.141 T -Sequence S 150 0.410 0.021 . -Sequence S 151 0.458 0.036 . -Sequence S 158 0.358 0.025 . -Sequence T 163 0.334 0.134 . -Sequence S 166 0.224 0.024 . -Sequence S 186

11、 0.192 0.026 . -Sequence T 188 0.299 0.446 . -Sequence T 192 0.431 0.036 . -Sequence S 197 0.426 0.042 . -Sequence T 202 0.541 0.551 T -Sequence T 203 0.538 0.449 T -Sequence S 204 0.445 0.187 . -Sequence T 206 0.586 0.485 T -Sequence T 209 0.555 0.049 T -Sequence T 210 0.563 0.082 T -Sequence S 216

12、 0.472 0.038 . -Sequence T 227 0.356 0.220 . -Sequence T 244 0.248 0.075 . -Sequence T 246 0.198 0.045 . -Sequence T 256 0.143 0.047 . -Sequence T 267 0.105 0.062 . -Sequence S 285 0.076 0.042 . -Sequence S 288 0.090 0.031 . -Sequence T 293 0.124 0.029 . -Sequence S 303 0.086 0.040 . -Sequence S 307

13、 0.078 0.020 . -Sequence S 313 0.066 0.051 . -Sequence S 330 0.084 0.054 . -Sequence T 340 0.129 0.054 . -Sequence T 352 0.179 0.074 . -Sequence T 358 0.206 0.049 . -Sequence T 361 0.229 0.034 . -Sequence S 365 0.156 0.020 . -Sequence S 369 0.162 0.031 . -Sequence S 371 0.169 0.066 . -Sequence S 376

14、 0.124 0.034 . -Sequence S 380 0.113 0.034 . -Sequence S 402 0.059 0.022 . -Sequence S 405 0.056 0.075 . -Sequence T 434 0.138 0.054 . -Sequence T 436 0.155 0.046 . -Sequence S 449 0.190 0.020 . -Sequence T 451 0.257 0.087 . -Sequence S 452 0.153 0.138 . -Sequence T 462 0.280 0.035 . -Sequence T 464

15、 0.281 0.049 . -Sequence S 481 0.102 0.052 . -Sequence T 495 0.343 0.076 . -Sequence S 496 0.267 0.035 . -Sequence T 509 0.671 0.465 T -Sequence T 510 0.677 0.267 T -Sequence S 511 0.570 0.305 S -Sequence T 514 0.700 0.239 T -Sequence S 518 0.546 0.187 S -Sequence T 522 0.710 0.069 T -Sequence T 524

16、 0.705 0.083 T -Sequence T 525 0.706 0.686 T -Sequence T 527 0.685 0.694 T -Sequence S 536 0.493 0.037 . -Sequence T 537 0.622 0.448 T -Sequence S 548 0.396 0.033 . -Sequence S 579 0.331 0.077 . -Sequence S 588 0.175 0.041 . -Sequence S 601 0.236 0.020 . -Sequence S 602 0.248 0.038 . -因此DNA序列不含信號(hào)肽序列

17、，所以它不能進(jìn)行O型糖基化修飾。六 磷酸化位點(diǎn)預(yù)測.S.S.S.S.S.S.Y 80.T.S.S.T.SY.S.SS.S.T.TSS.S. 160.Y.S.T.T.S.T.T.TT.S.T. 240.T.S. 320.S.S.S.S.S. 400.TS.T.T. 480.S.T.T.S.T. 560.S. 640 Phosphorylation sites predicted:Ser: 29Thr: 18Tyr: 3（Serine：29 Threonine：18 Tyrosine：3）結(jié)果：磷酸化位點(diǎn)：絲氨酸29 蘇氨酸18 酪氨酸3七蛋白功能域的分析和預(yù)測八蛋白序列二級(jí)結(jié)構(gòu)分析MSHTASS

18、CQELVENCAVHVAGMAQEDSRRGQVPSSFYHGANQELDLSTKVYKRESGSPYSVLVDTKMSKPccccccchhhhhhhhhhhhccccchccccccccccccccccccccccceeeeecccccceeeeeccccccHLHETEEQPYFRETRAVSDVHAVKEDRENSDDTEEEEEEVSYKREQIIVEVNLNNQTLNVSKGEKGVSSQcccccccccchhhhhhhhccceecccccccccccchhhhhhhccceeeeeeecccceeeeccccccccccSKETPVLKTSSEEEEEESEEEATDDSN

19、DYGENEKQKKKEKIVEKVSVTQRRTRRAASVAAATTSPTPRTTccccceeecccccccccchhhcccccccccchhhhhhhceeeheeecchhhhhhhhhhhhhcccccccccRGRRKSVEPPKRKKRATKEPKAPVQKAKCEEKETLTCEKCPRVFNTRWYLEKHMNVTHRRMQICDKCGKKcccccccccccccccccccccccccccchhhhccccccccccccccceehhhhhhhchhhhhhhhcccccFVLESELSLHQQTDCEKNIQCVSCNKSFKKLWSLHEHIKIVHGYAEK

20、KFSCEICEKKFYTMAHVRKHMVAeeeeeeecccccccccccceeecccchhhhhhhhhhhheeecccchhhchhhhhhhhhhhhhhhhhhhhhHTKDMPFTCETCGKSFKRSMSLKVHSLQHSGEKPFRCENCDERFQYKYQLRSHMSIHIGHKQFMCQWCGKccccccccccccchhhhhhhheeeeeecccccccccccccchhhhhhhhhhhcheeeecchhheheccccDFNMKQYFDEHMKTHTGEKPFICEICGKSFTSRPNMKRHRRTHTGEKPYPCDVCGQRFRFSNMLKAHKEKcccch