740-Y-P-740YPDGFR-DataSheet-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemE740 Y-PCat. No.: HY-P0175CAS No.: 1236188-16-1Synonyms: 740YPDGFR; PDGFR 740Y-P分式: CHNOPS分量: 3270.7Sequence: Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys-Ser-Asp-Gly-Gly-PO2-Tyr-Met-Asp-Met-SerSequence Shorten

2、ing: R-Q-I-K-I-W-F-Q-N-R-R-M-K-W-K-K-S-D-G-G-PO2-Y-M-D-M-S作靶點: PI3K作通路: PI3K/Akt/mTOR儲存式: Powder -80C 2 years-20C 1 yearIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實驗 H2O : 20 mg/mL (6.11 mM; Need ultrasonic)Mass Solvent1 mg 5 mg 10 mg Concentration制備儲備液1 mM 0.3057 mL 1.5287 mL 3.0574 mL5 mM 0.0611 m

3、L 0.3057 mL 0.6115 mL10 mM - - -請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液，并請注意儲備液的保存式和期限。BIOLOGICAL ACTIVITY物活性 740 Y-P (PDGFR 740Y-P)是有效，可滲透細胞的 PI3K 活化物。1/2 Master of Small Molecules 您邊的抑制劑師www.MedChemEIC50 & Target PI3K體外研究 740 Y-P is a synthetic biotinylated peptide consisting of the 16-amino acid sequence de

4、rived from the thirdhelix of the Antennapedia protein in tandem with a 9 amino acid phosphopeptide mimetic of the high affinityp85 binding site on the PDGF receptor. 740 Y-P readily binds GST fusion proteins containing both the N- andC- terminal SH2 domains of p85. 740 Y-P fails to bind GST alone, o

5、r a GST fusion protein containing the N-terminal SH2 domain of PLCg and shows relatively weak binding of a GST fusion proteins containing theSH2 domain of Grb2. The 740 Y-P peptide is also able to specifically interact with p85 in cell lysates asshown by affinity precipitation. 740 Y-P stimulates mi

6、togenesis at the lowest concentration tested (1 mg/mL).The peptide stimulates mitogenesis in both the presence and absence of serum (0.5%), and in the formerinstance a maximal response observed at 50 mg/mL. 740Y-P peptide to stimulate mitogenesis is highlyspecific and not a general feature of a cell

7、 permeable SH2 domain binding peptides 1.體內(nèi)研究 740 Y-P is not only internalised in living cells but can also interact with p85 in vivo. 1PROTOCOLCell Assay 1 Following serum starvation, C2 cells are cultured in the presence of the PDGFR 740Y-P peptide at the givenconcentrations (0, 1, 10, 100 g/mL) f

8、or 48 h. The mitogenic response is assessed by measurement of thepercentage of cells in S-phase 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.戶使本產(chǎn)品發(fā)表的科研獻 Biomaterials. 2019 Feb;194:57-72. Acta Biomater. 2018 Nov;81:278-292. Life Sci. 2019 Jul 25:116696.See more customer validations on HYPERLINK / www.MedChemEREFERENCES1. Derossi D, et al. Stimulation of mitogenesis by a cell-permeable PI 3-kinase binding peptide.McePdfHeightCaution: Product has not been fully validated for medical appl