Acetylcysteine-N-Acetyl-L-cysteine-DataSheet-生命科學(xué)試劑-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEAcetylcysteineCat. No.: HY-B0215CAS No.: 616-91-1Synonyms: N-Acetyl-L-cysteine; LNAC; NAC分式: CHNOS分量: 163.19作靶點: Endogenous Metabolite; ROS作通路: Metabolic Enzyme/Protease; Protein Tyrosine Kinase/RTK儲存式: Powder -20C 3 years4C 2 y

2、earsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實驗 DMSO : 130 mg/mL (796.62 mM)H2O : 50 mg/mL (306.39 mM; Need ultrasonic)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制備儲備液1 mM 6.1278 mL 30.6391 mL 61.2783 mL5 mM 1.2256 mL 6.1278 mL 12.2557 mL10 mM 0.6128 mL 3.0639

3、 mL 6.1278 mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液，并請注意儲備液的保存式和期限。體內(nèi)實驗請根據(jù)您的實驗動物和給藥式選擇適當?shù)娜芙獍?，配制前請先配制澄清的儲備液，再依次添加助溶?為保證實驗結(jié)果的可靠性，體內(nèi)實驗的作液，建議您現(xiàn)現(xiàn)配，當天使；澄清的儲備液可以根據(jù)儲存條件，適當保存；以下溶劑前的百分 指該溶劑在您配制終溶液中的體積占)：1. 請依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.17 mg/mL (13.30 mM); Clear solution2. 請依序添加每種溶

4、劑： 10% DMSO 90% (20% SBE-CD in saline)1/3 Master of Small Molecules 您邊的抑制劑師www.MedChemESolubility: 2.17 mg/mL (13.30 mM); Clear solution3. 請依序添加每種溶劑： 10% DMSO 90% corn oilSolubility: 2.17 mg/mL (13.30 mM); Clear solutionBIOLOGICAL ACTIVITY物活性 Acetylcysteine種粘液溶解劑，可以減少粘液的 度。Acetylcysteine 種 ROS 抑制劑 1

5、。IC50 & Target Human Endogenous Metabolite體外研究 Acetylcysteine prevents apoptotic DNA fragmentation and maintains long-term survival in the absence ofother trophic support in serum-deprived PC12 cells. Acetylcysteine also prevents death of PC12 cells andsympathetic neurons 2. Acetylcysteine causes do

6、se-dependent reductions in viability in rat and humanaortic smooth muscle cells 3. Acetylcysteine activates the Ras-extracellular signal-regulated kinase (ERK)pathway in PC12 cells. Acetylcysteine protects neuronal cells from death evoked by withdrawal of trophicsupport. Acetylcysteine increases nit

7、ric oxide (NO) release from protein-bound stores in vascular tissue.Acetylcysteine pretreatment of PC12 cells interferes with NGF-dependent signaling and neurite outgrowth,and it is suggested that Acetylcysteine interferes with redox-sensitive steps in the NGF mechanism 4.體內(nèi)研究 Acetylcysteine (150, 3

8、00 mg/kg) treatment significantly reduces liver transaminases in all groups oftreatment, mostly in group Acetylcysteine 300. Lung glutathione peroxidase is significantly increases in groupAcetylcysteine 300 (P=0.04), while the other oxidation biomarkers show no significant differences 6.Acetylcystei

9、ne improves cognition of 12-month-old SAMP8 mice in both the T-maze footshock avoidanceparadigm and the lever press appetitive task without inducing non-specific effects on motor activity,motivation to avoid shock, or body weight 5.PROTOCOLCell Assay 2 For survival experiments, washed cells are resu

10、spended in RPM1 1640 medium and plated in 0.5 mL at adensity of 8-10105 per well in 24 well plastic culture dishes coated with rat tail collagen. To feed, but toavoid loss of floating cells, fresh medium (0.2 mL) is added to the cultures on days 1, 5, and 10. Forexperiments involving primed PC12 cel

11、ls, cultures are pretreated for l-2 weeks with NGF in RPM1 1640medium supplemented with 1% heat-iN-acetylcysteinetivated horse serum. The cells are then washed andpassaged into serum-free RPM1 1640 medium.MCE has not independently confirmed the accuracy of these methods. They are for reference only.

12、Animal Rats: Rats are randomLy allocated into five groups: sham group (n=5), control group with IIR (n=8) and threeAdministration 6 groups with IIR who are given Acetylcysteine in different dosages: 150 mg/kg intraperitoneally 5 min beforeischemia (n=8, group Acetylcysteine 150), 300 mg/kg i.p 5 min

13、 before ischemia (n=7, group Acetylcysteine300), and 150 mg/kg i.p 5 min before ischemia plus 150 mg/kg 5 min before reperfusion (n=7, groupAcetylcysteine 150 + 150). After 4 h of reperfusion, the animals are euthanized by exsanguination from theabdominal aorta.2/3 Master of Small Molecules 您邊的抑制劑師w

14、ww.MedChemEMCE has not independently confirmed the accuracy of these methods. They are for reference only.戶使本產(chǎn)品發(fā)表的科研獻 Cell Rep. 2019 Jul 9;28(2):512-525.e6. Redox Biol. 2019 Jun;24:101215. Cell Prolif. 2019 May. Oxid Med Cell Longev. 2019 Mar. Sci Rep. 2016 Oct 27;6:35995.See more customer validatio

15、ns on HYPERLINK / www.MedChemEREFERENCES1. Halasi M, et al. ROS inhibitor N-acetyl-L-cysteine antagonizes the activity of proteasome inhibitors. Biochem J. 2013 Sep 1;454(2):201-8.2. Ferrari G, et al. N-acetylcysteine (D- and L-stereoisomers) prevents apoptotic death of neuronal cells. J Neurosci. 1

16、995 Apr;15(4):2857-66.3. Tsai JC, et al. Induction of apoptosis by pyrrolidinedithiocarbamate and N-acetylcysteine in vascular smooth muscle cells. J Biol Chem.1996 Feb 16;271(7):3667-70.4. Yan CY, et al. Prevention of PC12 cell death by N-acetylcysteine requires activation of the Ras pathway. J Neu

17、rosci. 1998 Jun1;18(11):4042-9.5. Farr SA, et al. The antioxidants alpha-lipoic acid and N-acetylcysteine reverse memory impairment and brain oxidative stress in agedSAMP8 mice. J Neurochem. 2003 Mar;84(5):1173-83.6. Kalimeris K, et al. N-acetylcysteine ameliorates liver injury in a rat model of intestinal i