NCBI序列提交方法

1、NCBI序列提交方法NCBI提交序列有兩大類(lèi)方式，一個(gè)是批量提交一個(gè)是單個(gè)序列提交。下面說(shuō)的就是單個(gè)序列提交方式，這是最新的提交方式，由于NCBI更新很快，也許過(guò)段時(shí)間又不一樣了，主要是按鈕位置可能會(huì)變。首先要注冊(cè)一個(gè)自己的NCBI賬號(hào)：點(diǎn)擊此處注冊(cè)然后登陸賬號(hào)：在已登陸狀態(tài)下點(diǎn)擊Submit點(diǎn)擊圖中標(biāo)記處選擇GengBank：submissionvviiareiimKipUgunngeuiwitre叨siarTrysrrtslQuitKStartYoifknow“he傳youwarmmgo.Seieccr.nohlblaReaseleclkxi選擇：Banklt：Howtosubmitdat

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9、esnyquesUans-ztculusngthe日;ankdt5equ=r:esubmESJonIdoIple-ueccrfcaclGwiEankUserserrkesal7-1N1.B1uHUJ赳1HLargloglw.P|而釧tnnlEnhqcy-andleeEnnkffandyourB4nkrtID#inItesubletIneuse序whoMeexistingNCBIPD時(shí)。由動(dòng)效的uwthesag=3*曲Z端心附小w.grtihig。中仲號(hào)umeme日ndPanoraglnOncflaajbmmer/加廂tou的Bar*H.IlwcornaetirWHinon電&ar領(lǐng)end已川L

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11、rexample-IS-rRNAgmalKITSiflNA,amoEqtb0s4iBkoraamslbakhor修府ent曬喇c既-youmWwusaiwwdMmBSlonwontKlealxreanmncnftirynurssqucncEsennlcarkalEdfoidenotnuireadvancedsequenceanasisiQDb：U歸Tr日IE#FEFNAjfeffiT與T口muluru?K：fTHai由U%IKUIM-1點(diǎn)擊NewSub,下面是你提交的記錄，即使你提交過(guò)程中中斷了，也可以Continue!以下是具體提交過(guò)程：一.填寫(xiě)基本信息?？贓GfWepenceSequcfr

12、rrgTechnohgMudK*deCigamsinSdimisswnCak?gQySojfcMSubmigion#1895945ContactInformatinrFirstName1khuphji|LastName|nu日啊|iDpjirtmentlenuzhou加”遇啊IhitituteriiSchoolafBio&FoodEnqineerln-q凱setAMrm|Fen?feAvenue咽忙J1UZIK4J|Seal制FrBlnGe|AnnulWIPJPaEtSlCifide|23EK1DD|Cwntiy回inaPhone1820515411:|山面邈逾械空匠ooi-KO-DOWMio

13、nab,*加EmailIncilhMprovideanemailaddrstoensure-1hatmessagesantrwewerlAtterruleEmil.圖中1是作者名字，可以添加作者名，F(xiàn)irst和LastName,填了就可以，其他可以不填。.圖中2是填是否出版，有三個(gè)選項(xiàng)：未出版，正在出版，已經(jīng)出版。需要注意的是，如果選則未出版，需要在Title中注明，否則無(wú)法提交。.可以添加作者，然后Continue即可。SequenceAuthor;FirstNamewii。加inmai同mtN&mesunixRemoveAAA|AA口因/dc|moresequenceauthors.Ref

14、erenceInformation#1Dleaseprovide1hetitleandrelevanitpublicatiordetails(valLine.issue.etcqJapaperlb?PUBLICATIONSTATUSllnpubfishuCIn-PressOReferencentieREFERENCEAUTHORSSameAsSequenceAuthors2SpecifNewAuthcfsAdcNnotri6ReTensncejKwhithareusdtodescriDeeachseqiBnce.stiould&eincludedanllheFASTAtarmat.11297r

15、Howmanydyouintendtas#ndinthis,submission?OSPIP2-1acaagctctgCgctcagctaagctctccaccattgcttgcaaagctgaGTCGTGTCACTCCTCCCGTTMGCTTCCCGCGGCGGCGAGAGCMGCTAAGCTAGGTCGGGCATGGGGAAGGACGAGGTGATGGAGAGCGGCGGCGCCGCCGGCGAGTTCGCGGCCAAGGACTACACGGACCCGCC7五填寫(xiě)你上傳序列所屬物種。然后即使繼續(xù)。六選擇你要你的序列式原始序列，就是你自己測(cè)序的序列，還是為已有序列做注釋。然后繼續(xù)。GenBan

16、kSubmissionscontactReferencesequencingTecrrKlogyNuclwtiaeorganismsubmissioncategorySubmission#1885945SubmissionCategoryndlcatewhetheryourseqwenceisananginalsubmissionora:hirdpartannotationsubinlss:Original口ipectlysequencedbysubmitzer.ThirdPartyAnnotationDenvedfromotherprimarysequencedata.Icuntinuslu

17、ntaclICopyriulit憎clairnfi=tcnsiGentertcw5chnoloarKInftrrnifltion.us電力。隨1HtfgrjoriMedkeUDRgEKPiW,日時(shí)鶯3dimUSA2CSQ4七序列信息填寫(xiě)你基因所屬細(xì)胞器填寫(xiě)你對(duì)基因的注釋?zhuān)梢蕴砑舆x項(xiàng)，根據(jù)自己實(shí)際情況添加。最下面可以添加你的PCR引物，然后繼續(xù)，會(huì)讓你檢查，你檢查后繼續(xù)即可。benUankSubmissionsContaclRetefenceSequencingrecnnoicgkNucleotKleQig0nlsm刖EiswonCategorysourceMoflitHSubmission#

18、1885945SourceModifiersSourceOrganelleiTaDaticinInfDrmationChooðeMnret.t0gariEiieolocal町n.rfapplicablernMizhandirianSourceMladahersChoosesourcemodiner(s)andentervaiiesherePleaserefErtoSchjiteEodif舊rliffordescnphnnandfcrmatspurcerngdifitrv削口向/mov,BiOm磯舊7haet|為除idanother占口M匚日modifierAnolhersourcerel

19、aledintcfniation?網(wǎng)ForexampleTaKonomirClaEsifncatkinorLineageforunftnjown/unirECognizedargani&msYoushouldcrsaku&enote1asmeheaderlabel.D。netenterindruidualsourcemodiilers-asplaintexlhere.ChooselhemTrotITyouwanttoincludePCRprimersi向yoursubmission,theckh父已ennMniiid八信息補(bǔ)充填加序列信息，可以上傳文件，也可以選項(xiàng)填寫(xiě)，建議用選項(xiàng)。選擇你的信息

20、，可以添加，然后繼續(xù)Ples&epirovidefeatureannotationsfcryQursubmissicntychoosingomeofth-etwoQAddfeaturesbyuploadmgnvecolumnfeaturetablefie*Addfeatures地，compllelflnginputformsThismethodis&estsuitablefor:asinglefeatureorafewteaturesappliedonasinglesequencethesamesinglefeatureorthesamefewfea1ure-&appliedtoallsequ

21、encesirFEdtue：5canbeaddedacrossanentiresequence口byspecificintervalswitoneormorequalifierscanbechosentoapplytoeachfeature,ifitisnotelecToaddafeltire.selectfeature匚alEqciryndtearturelypewithinthartcategotti：,codingRegkn(CDS)/GeneJmRNAityoursequenceencodesaprotein.X1RNA(rRNA,tRNA,non-codongRNA,mi5C_RNA

22、,etc)|EESSEng曰iRg7ORepeatregion(forsequencere-pe-ats,mobileelementsandsatelllrtes)ORegulatoryfeature(promoter,TATA_signal,RBSetc.)OOtnerAddAddedFeaturesforedining/irerricivalNofeaturesenteredyel|Cdnlind&在上圖中選擇添加選項(xiàng)，會(huì)讓你填寫(xiě)模板是正鏈還是負(fù)鏈，如果是部分序列，是哪一端，產(chǎn)物是指你把序列通過(guò)BlastP比對(duì)后的到的序列號(hào)(qualifiers)和相似度(Value),然后Accept。跳