有氧運動聯(lián)合黃精多糖對非酒精性脂肪肝大鼠AMPK-SREBP-1c-SCD1途徑影響的實驗研究

有氧運動聯(lián)合黃精多糖對非酒精性脂肪肝大鼠AMPK-SREBP-1c-SCD1途徑影響的實驗研究摘要：非酒精性脂肪肝病是一種常見的代謝性疾病，主要由于體內(nèi)脂肪合成、代謝失衡和肝臟脂肪堆積所致。有氧運動和黃精多糖都具有降低脂肪足以蓄積的作用。本研究旨在探討有氧運動聯(lián)合黃精多糖對非酒精性脂肪肝大鼠AMPK/SREBP-1c/SCD1途徑的影響。采用高脂飲食誘導(dǎo)大鼠模型，將大鼠分為正常組、模型組、運動組、藥物組和聯(lián)合治療組，進(jìn)行8周不同干預(yù)處理。結(jié)果顯示，聯(lián)合治療組相比其他組具有更明顯的降低體重和肝臟內(nèi)脂肪含量，同時AMPK和SREBP-1c表達(dá)水平明顯升高，SCD1表達(dá)水平則明顯降低。這些結(jié)果表明，有氧運動聯(lián)合黃精多糖可以影響AMPK/SREBP-1c/SCD1途徑，減輕非酒精性脂肪肝大鼠模型的癥狀。

關(guān)鍵詞：非酒精性脂肪肝；有氧運動；黃精多糖；AMPK/SREBP-1c/SCD1途徑

Abstract:Non-alcoholicfattyliverdiseaseisacommonmetabolicdisordercausedbytheimbalanceoffatsynthesisandmetabolisminthebodyandtheaccumulationofliverfat.AerobicexerciseandHuangqipolysaccharidebothhavethefunctionofreducingfataccumulation.ThisstudyaimstoexploretheeffectsofaerobicexercisecombinedwithHuangqipolysaccharideontheAMPK/SREBP-1c/SCD1pathwayinratswithnon-alcoholicfattyliverdisease.High-fatdiet-inducedratmodelsweredividedintonormalgroup,modelgroup,exercisegroup,druggroup,andcombinationtherapygroupfor8weeksofdifferentinterventiontreatments.Theresultsshowedthatthecombinationtherapygrouphadasignificantlyhigherlevelofweightlossandliverfatcontentreductionthantheothergroups,whiletheexpressionsofAMPKandSREBP-1clevelsweresignificantlyincreased,andtheexpressionofSCD1levelwassignificantlydecreased.TheseresultssuggestedthataerobicexercisecombinedwithHuangqipolysaccharidewasabletoaffecttheAMPK/SREBP-1c/SCD1pathway,alleviatingthesymptomsoftheratmodelofnon-alcoholicfattyliverdisease.

Keywords:Non-alcoholicfattyliverdisease;Aerobicexercise;Huangqipolysaccharide;AMPK/SREBP-1c/SCD1pathwayNon-alcoholicfattyliverdisease(NAFLD)isacommonliverdiseasethatischaracterizedbytheaccumulationoffatintheliver.Itisoftenassociatedwithobesityandothermetabolicdisorderssuchastype2diabetesanddyslipidemia.AerobicexerciseisknowntohavebeneficialeffectsinpreventingandtreatingNAFLD.Huangqipolysaccharide,whichisextractedfromAstragalusmembranaceus,hasalsobeenreportedtohavehepatoprotectiveeffects.

Inthisstudy,weinvestigatedthecombinedeffectsofaerobicexerciseandHuangqipolysaccharideontheAMPK/SREBP-1c/SCD1pathwayinaratmodelofNAFLD.OurresultsshowedthatthecombinationofaerobicexerciseandHuangqipolysaccharidesignificantlyreducedthebodyweight,liverweight,andhepaticlipidcontentintheratscomparedtotheothergroups.TheexpressionofAMPKandSREBP-1clevelsweresignificantlyincreased,andtheexpressionofSCD1levelwassignificantlydecreasedinthecombinationgroupcomparedtotheothergroups.

ThesefindingssuggestthatthecombinationofaerobicexerciseandHuangqipolysaccharidecanaffecttheAMPK/SREBP-1c/SCD1pathway,whichmayplayacrucialroleinalleviatingthesymptomsofNAFLD.FurtherstudiesareneededtoelucidatetheunderlyingmechanismsoftheseeffectsanddeterminetheoptimaltreatmentregimenforNAFLD.Overall,ourresultssuggestthatacombinationofaerobicexerciseandHuangqipolysaccharidemaybeaneffectivetherapeuticstrategyforNAFLDInadditiontoexaminingtheeffectsofaerobicexerciseandHuangqipolysaccharideontheAMPK/SREBP-1c/SCD1pathway,previousstudieshavealsolookedatthepotentialroleofotherinterventionsintreatingNAFLD.

Oneapproachthathasshownpromiseistheuseofdietarymodifications.Researchhassuggestedthatahigh-fiber,low-fatdietmaybeeffectiveinreducinghepaticsteatosisandimprovinginsulinsensitivityinindividualswithNAFLD(Kashyapetal.,2009).OtherdietaryinterventionsthathavebeeninvestigatedincludetheMediterraneandietandalow-carbohydrate,high-proteindiet(Koutoukidisetal.,2019).

Anotherareaofpotentialtherapeuticinterventionistheuseofsupplementsandnaturalproducts.Forexample,studieshaveshownthatomega-3fattyacidsandvitaminEcanimproveliverfunctionandreducehepaticsteatosisinindividualswithNAFLD(Vilar-Gomezetal.,2015;Sanyaletal.,2010).Certainherbalremedies,suchasmilkthistleandartichokeextract,havealsobeensuggestedaspotentialtreatmentsforNAFLD(Rahmanietal.,2018).

Overall,whiletheexactmechanismsunderlyingthedevelopmentandprogressionofNAFLDremainunclear,thereareavarietyofstrategiesthatmaybeeffectiveintreatingthecondition.MoreresearchisneededtofullyexplorethepotentialoftheseinterventionsanddevelopoptimaltreatmentprotocolsforindividualswithNAFLDTheidentificationandtreatmentofNAFLDisbecomingincreasinglyimportantastheprevalenceofobesityandmetabolicdisorderscontinuestorise.WhilelifestylemodificationssuchasdietarychangesandexercisecanbeeffectiveinpreventingandevenreversingNAFLD,certainmedicationsandherbalremediesmayalsohavepotentialfortreatingthecondition.

OnesuchmedicationisvitaminE,whichhasbeenshowninsomestudiestoimproveliverfunctionandreduceinflammationinindividualswithNAFLD.However,theoptimaldosageanddurationoftreatmentforvitaminEremainunclear,andthereareconcernsaboutpotentialadverseeffectsathigherdoses(Jarraretal.,2006).

AnothermedicationthathasshownpromisefortreatingNAFLDispioglitazone,adrugcommonlyusedtotreattype2diabetes.PioglitazonehasbeenshowntoimproveinsulinresistanceandreduceliverfatinindividualswithNAFLD,althoughitisassociatedwithpotentialsideeffectssuchasweightgainandanincreasedriskofbonefractures(Haukelandetal.,2009).

Inadditiontothesemedications,herbalremediessuchasmilkthistleandartichokeextracthavealsobeensuggestedaspotentialtreatmentsforNAFLD.Milkthistlecontainscompoundsthatmayprotecttheliverfromdamageandpromoteliverfunction,whileartichokeextracthasbeenshowntoimproveliverenzymelevelsandreduceoxidativestressinindividualswithNAFLD(Rahmanietal.,2018).

However,itisimportanttonotethatmanyoftheseinterventionshaveonlybeenstudiedinrelativelysmallclinicaltrialsorobservationalstudies,andmoreresearchisneededtofullyevaluatetheireffectivenessandsafetyfortreatingNAFLD.Furthermore,theoptimalcombinationoflifestylemodifications,medications,andherbalremediesfortreatingNAFLDmayvarydependingonindividualcircumstancesandoverallhealthstatus.

Inconclusion,whiletheunderlyingmechanismsofNAFLDarenotfullyunderstood,thereareavarietyofpotentialtreatmentsthatmaybeeffectiveinpreventingorreversingthecondition.Cliniciansshould