Thymidine-3-5-diphosphate-tetrasodium-Deoxythymidine-3-5-diphosphate-tetrasodium-DataSheet-生命科學(xué)試劑-MCE

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEThymidine3',5'-diphosphatetetrasodiumCat.No.:HY-115581ACASNo.:118675-87-9Synonyms:Deoxythymidine3′,5′-diphosphatetetrasodium;pdTptetrasodium分?式:C??H??N?Na?O??P?分?量:490.12作?靶點(diǎn):Apoptosis;MicroRNA作?通路:Apoptosis;Epigenetics儲存?式:4°C,sealedstorage,awayfrommoisture*Insolvent:-80°C,6months;-20°C,1month(sealed

storage,awayfrommoisture)溶解性數(shù)據(jù)體外實(shí)驗(yàn)H2O:50mg/mL(102.02mM;Needultrasonic)DMSO:3.33mg/mL(6.79mM;ultrasonicandwarmingandheatto60°C)MassSolvent1mg5mg10mgConcentration制備儲備液1mM2.0403mL10.2016mL20.4032mL5mM0.4081mL2.0403mL4.0806mL10mM0.2040mL1.0202mL2.0403mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；?旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲備液的保存?式和期限：-80°C,6months;-20°C,1month(sealedstorage,awayfrommoisture)。-80°C儲存時(shí)，請?jiān)?個(gè)?內(nèi)使?，-20°C儲存時(shí)，請?jiān)?個(gè)?內(nèi)使?。BIOLOGICALACTIVITY?物活性Thymidine3',5'-diphosphate(Deoxythymidine3′,5′-diphosphate)tetrasodium葡萄球核酸酶和含有1的都鐸結(jié)構(gòu)域(SND1，MicroRNA調(diào)控復(fù)合體RISC亞)與[3,5-2H2]酪氨酸核酸酶([3,5-2H2]tyrosylnuclease)的選擇性抑制劑。Thymidine3',5'-diphosphatetetrasodium具有抗腫瘤活性，在?化反應(yīng)也可?1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemE作催化劑。IC50&TargetStaphylococcalnucleaseandtudordomaincontaining1,SND1;[3,5-(2)H(2)]Tyrosylnuclease[1][2]體外研究Thymidine3',5'-diphosphatetetrasodium(200μM;18h)significantlyreducestheexpressionlevelofp65andp65nucleartranslocationinWTandAlb/SND1(specifictransgenicmouseoverexpressingSND1)hepatocytesbyinhibitingstaphylococcalnucleaseandtudordomaincontaining1(SND1)enzymeactivity.Thymidine3',5'-diphosphatetetrasodiuminhibitsthesphericalformationofWTandAlb/SND1hepatocytes[1].體內(nèi)研究Thymidine3',5'-diphosphatetetrasodium(0.8mg/kg;i.p.;twiceaweekfor4weeks)hasinsignificanteffectonserumliverenzymes(AST,ALT,AP),totalprotein(TP),albumin(Alb),andglobulin(Glo)inWTB6/CBAmice[1].Thymidine3',5'-diphosphatetetrasodium(0.8mg/kgand1.6mg/kg;i.v.;twiceaweekfor4weeks)significantlyinhibitstumorgrowthinWTB6/CBAmice[1].Thymidine3',5'-diphosphatetetrasodium(0.8,0.16and0.32mg/kg;s.c.;twiceaweekfor4weeks)inhibitstumorgrowth,inflammatoryreactionandtheexpressionoftumorinitiatingcells(TIC)markersinadultmaleNSGmice.Thymidine3',5'-diphosphatetetrasodiumup-regulatestheexpressionofPTEN,TGFBR2andCDKN1Capoptosisandselectivetumorsuppressorgenes[1].AnimalModel:2monthsoldWTB6/CBAmice[1].Dosage:0.8mg/kg.Administration:Intraperitonealinjection;twiceaweekfor4weeks.Result:Hadinsignificanteffectonserumliverenzymes,totalprotein,albuminandglobulinandshowedbiosafety.AnimalModel:NSGmice[1].Dosage:0.8,0.16or0.32mg/kg.Administration:Intravenousinjectionorsubcutaneousinjection;twiceaweekfor4weeks.Result:Inhibitedtumorgrowth,showedantitumoractivityandimmunomodulatoryeffects.REFERENCES[1].NidhiJariwala,etal.OncogenicRoleofSND1inDevelopmentandProgressionofHepatocellularCarcinoma.CancerRes.2017Jun15;77(12):3306-3316.[2].CohenJS,etal.Protonmagneticresonancestudiesofthetyrosineresiduesofstaphylococcalnucleaseusing[3,5-2H2]tyrosine[J].BiochimicaetBiophysicaActa(BBA)-ProteinStructure,1971,236(2):468-478.McePdfHeight2/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemECaution:Producthasnotbee