常見載體引物表

1、常見載體引物表VectorPrimer(F)Primer(R)PBADPBAD-F/TRXFPBAD-RpACTT7T3pAc5.1/V5-HisAc5-ProforBGHpACT2GAL4-AD-CforpGAL4-ADrvpAS2-1GAL4 BDpAS2-1.RpB42ADpB42ADFpB42ADRpBACPAK8BAC1BAC2pBK-CMVT7T3PBS(SK/KS)/M13-M13F/T7T3/M13RpBV220PBV220FPBV220RpCAMBIA 1301(1300)P1P2（同2.0-rev）pCAMBIA 2300M13R(-48)M13F(-47)PCANTB5E

2、S1/M13RS6pCAT3-enhancerRVP3此載體無反向引物pcDNA3.0CMV-F/T7SP6/BGHpcDNA3.1CMV-F/T7BGHpcDNA4T7/CMV-FBGHpcDNA6T7/CMV-F(J21025在T7前面）BGHpcDNAIIT7SP6pCE2.1M13FM13RpCEP4pCEP-FEBV-RpCF-TM13FM13RpCI T7（17Base）此載體無反向引物pCI-neoT7（17Base）T3pCMS-EGFPT7T3pCMV-3Tag-4AT3 /PFLAG-CMV-FT7pCMV5pCMV5FpCMV5RpCMV5-FlagpCMV5FpCMV5

3、RpCMV-MYC/NUCCMV-FBGHPCMV-HACMV-FEBV-RpCMV-SportM13F/T7SP6/M13RpCMV-Tag(KAN+)T3T7pCR2.1-TOPOM13F/T7M13RpCR3.1T7BGHpCS2SP6T7pDonarM13FM13RpDONR221M13FM13RpDriveT7SP6pDRIveR(KAN+)T7SP6pDsRED1-C1pDsRED-ex-C1-FpEGFP-N-3(距離很近,一般不用)pDsRed1-N1CMV-ProforRFP-NrevpDsRed-Monomer-N1CMV-ProforDSRED1_n_primerpDsR

4、ED2-C1(KAN+)pDsRED-ex-C1-FpEGFP-N-3pDSRED-N1(KAN+)pEGFP-N-5PDSRED-N-RpECFP-CpEGFP-C-5pECFP-C-3 pECFP-N1pEGFP-N-5pEGFP-N-3pEF/myc/ERpEF-FpCDNA3.1RpEGFP-C(KAN+)pEGFP-C-5pEGFP-C-3pEGFP-N(KAN+)pEGFP-N-5pEGFP-N-3pENTR/D-TOPO(KAN+)M13FM13RpET-*(His)(KAN+)T7T7 TerpET22(a,b,c)(AMP+)T7T7 TerpET28,30,24,49(KA

5、N+)T7T7 TerpET32(a,b,c)(AMP+)T7/S.tagT7 TerpET-42a (-b, -c)(+)StagT7 TerpET50(amp+)T7/s.tagT7TERpET44(AMP+)T7/s.tagT7 TerpEYFP-N1pEGFP-N-5pEGFP-N-3PETBluePETBlueT7UP(太靠前，在T7前)/ PETBlueUPPETBlueDOWNpEYFP-C1pEGFP-C-5pEGFP-C-3 pFLAG-CMV CMV30CMV-24pGAD424 GAL4 AD3ADpGADGH GAL4 AD3ADpGADT7-RecGAL4 AD/T7

6、3ADpGAPZa-Aa-FACTOR 3AOXpGBKT7(Kana+)T73BDpGEM-T(-Easy)M13F/T7SP6/M13RpGEX-(*)TpGEX-4T-5pGEX-4T-3pGL2GLP1GLP2pGL3RVP3GLP2(RVP4)PinpointTM Vector Pinpoint PrimerSP6pIRES PIRES-F/T7T3/PIRES-R (J40720)pIRES2-EGFPpIRES2-EGFP.P5pIRES2-EGFP.P3pLXSN pLXSN-FpLXSN-RpMAL-c2EMalE primerM13F(-47)pMAL-C2xP5P3pMA

7、L-p2XMalE primerM13F(-47)PMD18-TM13R(-48)M13F(-47)PMD19-TM13F(-47)M13R(-48)pPIC9K(AMP+、ZEO+）5AOX/a-factor3AOXpPICZa5AOX/PPICZa-F3AOXpPROEXHTAM13R(-48)無反向引物pQE30or40pQE30-FpQE-R:pRSETT7T7TERpSilencer3.1-H1 hygroM13F(-47)3.0REVpSILENCEV2.0-U6T72.0REVpSK01-TM13FM13RpSK-CMV(KAN+)T7T3pSP72T7SP6pSport1SP6

8、/M13F(-47)T7/M13RpSUPERT7T3pTA2 M13F/T7(優(yōu)先使用M13F）T3/M13RpT-AdvM13F /T7M13RpTARGET TMpTarget.F(在T7前面）/T7M13R?pTO-T7T7T7TERpTRC99a-cpTRC99C-FpTRC99C-RpTriPLEx25pTriPLEx2T7pTWIN-1T7T7TER(客戶確認(rèn)再用）pUC18(19)/118(119)M13F(-47)M13R(-48)pUC57M13R(-48)/M13RM13F(-47)/M13FpUCm-TM13F/T7M13RpVAX1T7BGHpWSK29T7/M13F

9、/M13F(-47)T3/M13R/M13R(-48)pXT7 T7SP6pYES2 T7pYes2.RpLEXApLEXA-FpLEXA-RpLNCXpLNCX-FpLNCX-RpRECEIVERCMV-F此載體無反向引物pSHUTTLE-CMVPSHUTTLE-CMV-FPSHUTTLE-CMV-RpSP64/65SP6此載體無反向引物pSTBlue-1T7SP6pT7Blue(R)T7M13F(-47)pRK5SP6pSG5-3 or SV40pA-RpMSCVMSCV5MSCV-revpBABEpBABE5pBABE3pFastBac HT_A (_B, _C)pFastBac-FpF

10、astBac-RpFastBac1PH-Profor/BaculoVforpFastBac-RpACYCDuet-1 (MCS I)ACYCDuetUP 1DuetDOWN1pDSRED2-N1(KAN+) pEGFP-N-5DsRed1-NpFastBac Dual (MCSI)BaculoVfor/PHpFastBac-R/SV40-pArevpBSN-TT7T3pHISi1PHISi-1無pRSV-Rev(根據(jù)酶切位點(diǎn)選擇不同引物)pREPF/M13FHIV-Rev-NES/ pGEX-3EZ-TM13F(-47)M13R(-48)pMSCV-hygMSCV5PMSCV-R/ MSCV-

11、revPTHIOHIS (A )TRXF(new)M13FPDEST22PDEST22-FPDEST22-RpLXSNpLXSNfwpLXSNrvpLEXApLEXA-FpLEXA-RpCDHpCDH-EF1無PVXLBALBBpAcGP67 (_A, _B, _C)PHpAcGP67rvpAD-TrackpAD-Track-F無PLPCPLPC-F/CMV-FPLPC-RpHAGE-CMV-MCS-IZsGreenpHAGE-CMV-F暫沒設(shè)計(jì)PLENTI-HISCMV-F無pDSRED-PRESS-C1pDSRED -C1-FpDSRED-C1-RpCR-BluntM13RM13F/T7pMT/V5-His_A (_B,_C)MT-ProforBGHrevpEASY-T3T7/M13FSP6/M13RPSILENCE-1.0-U6T72.0REV(同P2(pCAMBIA 1301)pLVX-IRES-ZsGreen1PIRES2-EGFP-FPIRES2-EGFP-RpDC316pDC316FpDC316RPMK0.1PLXSN-5PL