限制酶圖表資料

1、限制酶圖表 WORD文檔使用說(shuō)明：限制酶圖表 來(lái)源于PDFWORDPDF轉(zhuǎn)換成WROD 本W(wǎng)OED文件是采用在線轉(zhuǎn)換功能下載而來(lái)，因此在排版和顯示效果方面可能不能滿足您的應(yīng)用需求。如果需要查看原版WOED文件，請(qǐng)?jiān)L問(wèn)這里限制酶圖表 文件原版地址: 限制酶圖表|PDF轉(zhuǎn)換成WROD_PDF閱讀器下載圖表： 圖表：限制酶反應(yīng)條件EnzymeRecomended buffer for 100% activity BEnzyme activity in Fermentas buffers, % G O R (red) 1X 0-20 0-20 Tango? (yellow) 1X / 2X 100 N

2、R 20-50 50-100Tango?Units forThermalbuffer for overnight inactivation double incubation, digestion u/?g DNA in 20min(blue) (green) (orange) 1X 1X 1X 0-20 0-20AanI (PsiI)Tango?50-100 50-100 NR NR1X or 2X 2X (+oligo) 1X* 1X or 2X65° CAarIAarI +oligo(+oligo) (+oligo) (+oligo) (+oligo) (+oligo) (+o

3、ligo) 100 50-100 20-50 20-50 0-20 0-20 0-20 0-20 50-100* 100 0-20 20-5065° CAasI (DrdI) AatII Acc65I (Asp718I) AdeI (DraIII) AjiI (BmgBI)B Tango? 80° C 65° CO0-2020-5010020-5020-5050-100 1X or 2X65° CG AjiI0-20 NR 0-20100 NR 50-10020-50 20-50* 20-50100 NR 100100* NR20-50 1X* or 2

4、X 20-50* 2X* No 65° CAjuIR +SAM50-100 50-100 1X or 2X(+SAM) (+SAM) (+SAM) (+SAM) (+SAM) (+SAM) (+SAM) 0-20 0-20 0-20 100 0-20 20-50 2X65° CAlfIR +SAM(+SAM) (+SAM) (+SAM) (+SAM) (+SAM) (+SAM) (+SAM) 0-20 50-100 0-20 0-20 0-20 0-20 100 0-20 20-50 100 100 20-50 1X or 2X 1X or 2X65° CAloI

5、 AluI Alw21I (BsiHKAI) Alw26I Alw44I ApaI BamHI BauI (BssSI) BclI BcnI (NciI) BcuI (SpeI) BdaIR Tango? 65° C 65° CO0-2020-5010050-10020-5050-100 1X or 2X65° CTango? Tango? B BamHI Tango? G Tango? Tango? G +SAM50-100 50-100 100 20-50* 0-20 20-50 20-50100 100 20-50 100 50-100 100 50-100

6、0-20 0-20 0-20 20-50 0-20 20-50 50-100 0-20 0-200-20 50-100 0-20 50-100* 50-100 20-50 50-100 20-50100 100 20-50 100* 100 100* 100 1001001X or 2X 65° C 65° C 65° C 80° (10u) C 65° C 80° (10u) C 65° C No 65° C50-100 1X or 2X 0-20 1X50-100 1X* or 2X 50-100 1X or

7、2X 100 1X* or 2X50-100 1X or 2X 0-20 1X 1X*50-100 50-100 NR 10020-50 50-100* 50-100(+SAM) (+SAM) (+SAM) (+SAM) (+SAM) (+SAM) BfiI (BmrI) BfmI (SfcI) BfuI (BciVI) BglI BglII Bme1390I (ScrFI) BoxI (PshAI) BpiI (BbsI) Tango? Tango? BfuI O O 20-50 0-20 NR 0-20 0-20 20-50 50-100 NR 50-100 20-50 0-20 0-20

8、 0-20 100 100 0-20 0-20 0-20 100 50-100 100 100 NR 0-20 0-20 0-20 20-50 50-100* 100 100 1X 1X or 2X 2X* 2X 2X 65° C 65° C 80° C 65° C NoO20-5050-10010050-100 50-100 50-100 1X or 2X80° CTango? G0-20 20-50 0-200-20 100 20-500-20 50-100 0-2020-5010020-501X or 2X 80° C 65&#

9、176; C50-100 50-100 50-100 1X or 2X 0-20 100 20-50 1XBplITango?(+SAM) (+SAM) (+SAM) (+SAM) (+SAM) (+SAM) (+SAM) 1X* or 2X*65° CBpu10IBpu10I0-2020-50* 50-100*100*50-100*100*80° CBpu1102I (BlpI) BseDI (BsaJI) BseGI (BtsCI) BseJI (BsaBI) BseLI (BslI) BseMI (BsrDI) BseMII (BspCNI) BseNI (BsrI)

10、 BseSI (Bme1580I) BseXI (BbvI) Bsh1236I (BstUI)Tango?50-100 50-10020-5020-5010020-501X or 2X80° CTango?50-10020-500-200-2010050-100 1X or 2X80° CTango?20-5050-10020-5020-5010020-501X or 2X80° CONR100*100NRNR100*2X*NoTango?20-501000-2020-5010050-100 1X or 2XNoR0-2020-500-2010050-100 50

11、-100 1X or 2X80° CTango?50-100 50-10050-10050-10010050-100 1X or 2X(+SAM) (+SAM) (+SAM) (+SAM) (+SAM) (+SAM) (+SAM) 100 20-50 0-20 0-20 50-100 20-50 1X or 2X80° CB80° CG20-501000-2020-5050-1000-201X80° (10u) CBseXINRNRNRNRNRNRNR80° CR0-200-2050-10010020-5050-100 1X or 2X65&#

12、176; CBsh1285I (BsiEI) BshNI (BanI) BshTI (AgeI) Bsp119I (BstBI) Bsp120I (PspOMI) Bsp1407I (BsrGI) Bsp143I (Sau3AI) Bsp68I (NruI) BspLI (NlaIV) BspOI (BmtI) BspPI (AlwI) BspTI (AflII) Bst1107I (BstZ17I) BstXI Bsu15I (ClaI) BsuRI (HaeIII)G20-5010020-500-200-2020-502X65° CO O0-20 0-2020-50 20-501

13、00 10050-100 50-1000-20 20-50100 20-502X 1X or 2X 65° C 65° CTango?20-500-200-200-201001001X or 2X80° CB10020-500-2020-5050-1000-201X80° CTango?0-2020-500-2020-5010050-100 1X or 2X65° CBsp143I20-5020-500-200-2050-10020-501X or 2X65° CO0-2020-5010050-10020-5050-100 1X or

14、 2X65° CTango?50-100 50-1000-2020-5010020-501X or 2X65° CO0-200-201001000-2020-502X80° CTango? O20-50 0-2020-50 0-200-20 1000-20 20-50100 0-200-20 50-1001X 2X 80° C 65° CO20-5050-10010010020-501001X or 2X65° CO Tango?20-50 20-50100 20-50100 20-5050-100 50-100 20-50 1001

15、00 20-501X or 2X 1X or 2X 65° C 65° CR0-200-200-2010050-1001001X or 2X80° CBveI (BspMI)O +oligo0-2020-5010020-5050-1001001X or 2X (+oligo)(+oligo) (+oligo) (+oligo) (+oligo) (+oligo) (+oligo) 20-50 20-50 20-50 50-100 10065° CCaiI (AlwNI) Cfr10I (BsrFI) Cfr13I (Sau96I) Cfr42ITango

16、?50-100 1X or 2X65° CCfr10I0-2020-5020-5050-10020-5050-100 1X or 2XNoTango?50-100 50-10020-5020-5010020-501X or 2X65° CB10050-1000-200-2050-1000-201X65° C(SacII) Cfr9I (XmaI) CfrI (EaeI) CpoI (RsrII) CseI (HgaI) Csp6I (CviQI) DpnI DraI Eam1104I (EarI) Eam1105I (AhdI) Ecl136II (EcoICRI

17、) Eco105I (SnaBI) Eco130I (StyI) Eco147I (StuI) Eco24I (BanII) Eco31I (BsaI) Eco32I (EcoRV) Eco47I (AvaII) Eco47III (AfeI) Eco52I (EagI) Eco57I Cfr9I Tango? Tango? R 0-20 0-20 0-20 0-20 50-100 0-20 0-20 20-50 100 20-50 100 100 100* 0-20 0-20 1X 1X 65° C 65° C 65° C 80° (10u) C50-

18、100* 50-100 20-50 NR 50-10050-100 1X or 2X 50-100 1X* or 2X50-100* 50-100B10050-1000-200-2050-1000-201X65° CTango? Tango?10010050-100 20-5050-100 20-50100 10050-100 1X or 2X 50-100 1X or 2X 80° C 65° C50-100 50-100Tango?50-100 50-1000-200-201000-201X65° CEam1105I20-5050-1000-200-

19、2050-10020-501X or 2X65° CEcl136II50-10020-500-200-2050-1000-201X65° CTango?100*50-1000-200-201000-201X65° CO0-2020-5010050-100 50-1001001X or 2X65° CB10050-10020-5020-5050-1000-201X80° CTango?50-100 50-10020-5020-501000-201X65° CG50-1001000-200-2050-10020-501X or 2X65&

20、#176; CR0-2050-10050-10010020-501001X or 2X65° CR0-2050-10050-10010050-100 50-100 1X or 2X65° CO0-2020-5010010050-1001001X or 2X80° CEco52I0-200-200-2020-500-2020-502X65° CG +SAM10010020-5020-5050-100 50-100 1X or 2X65° C(AcuI)(+SAM) (+SAM) (+SAM) (+SAM) (+SAM) (+SAM) (+SAM)

21、 100 50-100 0-20 20-50 50-100 0-20 1XEco57MIB +SAM(+SAM) (+SAM) (+SAM) (+SAM) (+SAM) (+SAM) (+SAM)65° CEco72I (PmlI) Eco81I (Bsu36I) Eco88I (AvaI) Eco91I (BstEII) EcoO109I (DraII) EcoRI EcoRII EheI (SfoI) Esp3I (BsmBI)Tango?NRNR0-200-2010020-501X or 2X65° CTango?50-1001000-200-201000-201X8

22、0° CTango?10050-1000-200-2010020-501X or 2X65° CO20-5020-5010050-100 50-1001001X or 2X65° CTango?50-10020-5020-5020-501001001X or 2X65° CEcoRI O Tango?0-20 20-50 20-50 100NR 50-100 50-100 20-50100 100 0-20 0-20100* 50-100 0-20 0-20NR 20-50 100 1001002X 65° C 80° C 65

23、76; C50-100 1X or 2X 20-50 0-20 1X or 2X 1X (+DDT) 1XTango?(+DTT) (+DTT) (+DTT) (+DTT) (+DDT) (+DTT) 20-50 20-50 0-20 0-20 100 50-10065° CFaqI (BsmFI)Tango?(+SAM) (+SAM) (+SAM) (+SAM) (+SAM) (+SAM) (+SAM) 0-20 50-100 100 0-20 20-50 50-100 100 0-20 0-20 20-50 50-100 0-20 20-50 20-50 0-20 100 100

24、 100 50-100 0-20 2X 1X80° CFspAI FspBI (BfaI) GsuI (BpmI) HhaI Hin1I (BsaHI) Hin1II (NlaIII)O Tango? B Tango? 65° C 65° C 65° C 80° C50-100 1X or 2X 20-50 1X or 2X50-100 50-100G20-5010020-5020-5020-5020-501X or 2X65° CG50-100 20-50100 20-5020-50 0-2050-100 50-100 50-100

25、 1X or 2X 0-20 100 0-20 1X65° CHin4ITango?(+SAM) (+SAM) (+SAM) (+SAM) (+SAM) (+SAM) (+SAM)65° CHin6I (HinP1I) HincII (HindII) HindIIITango?50-100 50-10020-5020-5010050-100 1X or 2X65° CTango?50-100 50-10020-5050-10010050-100 1X or 2X65° CR0-2020-500-2010050-100 50-100 1X or 2X80&

26、#176; CHinfI HpaII HphI Hpy8I (MjaIV) HpyF10VI (MwoI) HpyF3I (DdeI) I-SceI Kpn2I (BspEI) KpnIR Tango? B0-2020-5050-100 0-20 0-20100 20-50 0-2050-100 50-100 1X or 2X 100 20-50 20-50 0-20 1X or 2X 1X 65° C 65° C 65° C50-100 50-100 100 0-20Tango?50-100 50-1000-2020-5010050-100 1X or 2X80

27、° CTango?0-200-200-200-2010050-100 1X or 2X80° CTango?20-5020-5020-5020-5010050-100 1X or 2X65° CTango?50-100 50-10050-10050-10010050-100 1X or 2X65° CTango?50-100 50-1000-2020-5010050-100 1X or 2X80° CKpnI20-500-200-200-2020-500-201X 1X* or 2X* 1X or 2X80° CKspAI (HpaI

28、)B10050-100*20-5020-50100*50-10065° CLguI (SapI) Lsp1109I (BbvI) LweI (SfaNI) MauBI MbiI (BsrBI) MboI MboII MlsI (MscI) MluI MnlI Mph1103I (NsiI) MreI (Sse232I) MspI (HpaII) MssI (PmeI)Tango?20-5050-10020-5020-5010020-5065° CLsp1109I0-2020-50* 50-100*10020-50* 20-50* 1X* or 2X65° CTan

29、go? Tango? Tango? R B R R G0-20 0-20 20-500-20 0-20 1000-20 0-20 0-20 50-100 20-50 0-20 50-100 20-5020-50 0-20 20-50 100 0-20 100 100 20-50100 100 100 50-100 50-100 20-50 20-50 20-5020-50 0-20 20-50 100 20-501X or 2X 1X 1X or 2X 1X or 2X 1X or 2X 65° C 65° C 65° C 65° C 65°

30、C 65° C 65° C 65° C50-100 50-100 100 0-20 0-20 50-100 50-100 20-50 20-50 10050-100 1X or 2X 50-100 1X or 2X 20-50 1X or 2XR0-2050-10020-5010050-100 50-100 1X or 2X65° CG0-201000-200-2050-1000-201X80° CTango? B50-100 50-100 100 0-200-20 0-200-20 0-20100 20-5050-100 1X or 2X 0

31、-20 1X 65° C 65° CMunI (MfeI) Mva1269I (BsmI) MvaI (BstNI) NcoI NdeI NheI NmuCI (Tsp45I) NotI NsbI (FspI) OliI (AleI) PacI PaeI (SphI) PagI (BspHI) PasI PauI (BssHII) PdiI (NaeI) PdmI (XmnI) PfeI (TfiI) Pfl23II (BsiWI) PfoI PpiI Ppu21I (BsaAI) PscI (PciI)G1001000-200-201000-201X65° CR

32、0-2020-5050-1001000-2050-1002X65° CR R O Tango? O Tango?20-50 0-20 0-20 20-50 0-20 10020-50 20-50 20-50 20-50 0-20 20-5050-100 20-50 100 20-50 100 0-20100 100 100 50-100 50-100 0-2020-50* 20-50 20-50 100 0-20 1001001X* or 2X 80° (10u) C 80° C 80° C 65° C 65° C 65°

33、C50-100 1X or 2X 50-100 1X or 2X 100 50-100 0-20 1X or 2X 2X 1XR0-2020-5050-10010020-5050-100 1X or 2X65° CO Tango? R R PacI B O PasI0-20 20-50 0-20 0-20 20-50 100 0-20 NR20-50 50-100 0-20 0-20 20-50 50-100 50-100 NR100 0-20 100 0-20 0-20 0-20 100 NR20-50 20-50 100 100 0-20 0-20 NR NR0-20 100 0

34、-20 0-20 0-20 50-100 NR NR20-50 20-50 50-100 50-100 0-20 0-20 NR NR2X 1X or 2X 2X 2X NR 1X NR NR 65° C 65° C 80° C 65° C 65° C 65° C 80° C 80° CR0-200-201001000-201002X80° CTango? Tango? O50-100 20-50 0-2020-50 50-100 20-500-20 0-20 1000-20 0-20 50-100100

35、 100 20-5050-100 1X or 2X 0-20 1X 65° C 65° C 65° C50-100 1X or 2XTango?20-5050-10020-5020-501000-201X65° CTango? R0-20 0-2020-50 0-2050-100 0-200-20 10010050-100 1X or 2X 65° C 65° C50-100 50-100 1X or 2XPpu21I50-100*100*20-50NRNRNRNR65° CTango?20-5020-500-200-201

36、000-201X65° CPsp1406I (AclI) Psp5II (PpuMI) PstI PsuI (BstYI) PsyI (Tth111I) PvuITango?10050-1000-2020-501000-201X65° CG0-2010020-5020-5050-1001001X or 2X80° CO B50-100 50-100 100 20-50100 0-20100 0-2050-100 50-100 1X or 2X 50-100 0-20 1X 80° (10u) C 80° CB10050-1000-200-205

37、0-1000-201X80° CR0-2020-5050-10010050-1001001X or 2X 1X* or 2X* 1X 1X or 2X 1X or 2X 2X80° (10u) CPvuIIG50-100*10020-5050-100 20-50* 20-50*80° CRsaI RseI (MslI) SacI SalI SatI (Fnu4HI) ScaI SchI (MlyI) SdaI (SbfI) SduI (Bsp1286I) SfaAI (AsiSI) SfiI SgeI SgrDI SgsI (AscI) SmaI SmiI (Sw

38、aI) SmoI (SmlI) SmuI (FauI) SsiI (AciI)Tango? R SacI O50-100 0-20 50-100 0-2020-50 50-100 20-50 0-200-20 50-100 0-20 1000-20 100 0-20 20-50100 20-50 50-100 0-200-20 100 20-50 50-100 65° C 65° C 65° C 65° CG20-5010020-5020-5050-10020-501X or 2X65° CScaI Tango? SdaI0-20 20-50

39、NR0-20 50-100 NR0-20 0-20 0-200-20 0-20 0-200-20 100 NR0-20 0-20 20-50NR 1X 2X 80° (10u) C 65° C 65° CSduINR50-100*0-200-20NRNRNR65° CTango? G SgeI R Tango? Tango? O Tango? Tango? O50-100 50-100 0-20 0-20 0-20 50-100 0-20 50-1000-20 100 0-20 0-20 0-20 0-20 0-20 20-500-20 20-50 0-

40、20 0-20 0-20 0-20 100 0-20 0-20 1000-20 0-20 NR 100 50-100 0-20 20-50 20-50 20-50 50-100100 100 NR NR 100 100 0-20 100 100 NR0-20 0-20 NR 1001X 1X NR 2X 80° C 80° C 65° C 65° C 65° C 65° C 65° C 80° C 65° C 65° C50-100 1X or 2X 0-20 20-50 20-50 20-50 100 1X 2X 1X or 2X 1X or 2X 2X50-100 50-100 NR 20-50SspDI (KasI) SspI TaaI (HpyCH4III) TaiI (MaeII) TaqI TasI (Tsp509I) TatI TauI Tru1I (MseI