銀杏葉制劑改善MID癡呆大鼠學(xué)習(xí)記憶障礙的實(shí)驗(yàn)研究

1、銀杏葉制劑改善MID癡呆大鼠學(xué)習(xí)記憶障礙的實(shí)驗(yàn)研究                         作者：鄭向紅，郭景財(cái)，雷雅梅 【關(guān)鍵詞】  多發(fā)性梗死性腦癡呆；大鼠；學(xué)習(xí)記憶障礙；銀杏葉摘要 目的：探討銀杏葉制劑對(duì)多發(fā)性梗死性腦癡呆(MID)大鼠學(xué)習(xí)記憶能力的影響，為臨床應(yīng)用提供實(shí)驗(yàn)依據(jù)。方法：建立MID動(dòng)物模型，對(duì)其進(jìn)行游水迷宮、傷害性電刺激

2、試驗(yàn)和海馬區(qū)乙酰膽堿(Ach)含量測(cè)定。結(jié)果：用藥各組大鼠游迷宮所需時(shí)間和傷害性電刺激出錯(cuò)次數(shù)顯著少于模型組(P<0.01)，除第1天記憶出錯(cuò)次數(shù)差異無(wú)顯著性外(P>0.05)，銀杏葉治療組在游水迷宮所需時(shí)間及出錯(cuò)次數(shù)上均明顯優(yōu)于西藥治療組(P<0.01,P<0.05)。MID大鼠海馬區(qū)Ach含量顯著降低(P<0.01),經(jīng)藥物治療后其含量顯著提高(P<0.01)，且銀杏葉治療組優(yōu)于西藥組(P<0.01)。結(jié)論：銀杏葉制劑能有效的改善MID大鼠學(xué)習(xí)記憶能力，同時(shí)明顯提高海馬區(qū)的Ach含量。關(guān)鍵詞 多發(fā)性梗死性腦癡呆；大鼠；學(xué)習(xí)記憶障礙；銀杏葉An Ee

3、xperimental Study of Preparation of Ginkgo Biloba Leaves on Learning and Memory Disorder in a Multiple Ischemic Dementia Rat ModelAbstract：Objective To explore the effect of preparation of ginkgo leaf on learning and memory disorder in Multiinfarct dementia rats,and provide experimental data for cli

4、nical application.Methods The models of multiinfarct dementia in rats were established,Ymaze test,nocuity electric stimulation test and the contents of acetylcholine(Ach) in hippocampus were measured.Results In each treated group,the time of Ymaze test and the frequency of the fault of nocuity elect

5、ric stimulation were significantly lower than the model group(P<0.01).Except the fault frequency at the first day,the time of Y maze test and the frequency of the fault of nocuity electric stimulation in ginkgo leaf preparation group were significantly higher than the western medicine group.Ach d

6、ecreased significantly in hippocampus of the MID dementia rats(P<0.01).Medication significantly upregulated Ach level,moreover ginkgo leaf preparation group was higher than the western group.Conclusion Ginkgo leaf preparation is effective in improving learning and memory dysfunction in multiinfar

7、ct dementiarats,it can also upregulate Ach content in hippocampus.Key words：Multiple Ischemic Dementia;Rat;Learning and memory disorder;Ginkgo leaf銀杏葉是一種具有多種藥理作用的中藥，黃酮總甙酐是從銀杏葉中提取的主要藥物成分。研究表明，黃酮總甙酐是一種天然的自由基清除劑，可以保護(hù)機(jī)體免受自由基的損害，改善腦循環(huán)和神經(jīng)元的功能障礙。它已廣泛應(yīng)用于治療冠心病和腦缺血，但關(guān)于銀杏葉制劑對(duì)認(rèn)知功能障礙的實(shí)驗(yàn)研究少見(jiàn)報(bào)道。本研究旨在探討銀杏葉制劑對(duì)MID大鼠學(xué)

8、習(xí)記憶能力的影響，初步研究其機(jī)制，為臨床合理應(yīng)用銀杏葉制劑提供一定的實(shí)驗(yàn)依據(jù)。1材料與方法1.1實(shí)驗(yàn)動(dòng)物選用體重350 g400 g，鼠齡10個(gè)月1 a，封閉群純種雄性SD大鼠30只(由西安交通大學(xué)醫(yī)學(xué)部實(shí)驗(yàn)動(dòng)物中心提供)。1.2藥物銀杏葉制劑(含總黃銅甙24%)由深圳海王制藥有限公司提供；對(duì)照組用西藥西得鎮(zhèn)(Hydergine,瑞士SANDOZ產(chǎn)品)，用時(shí)配成0.03 g/L懸液。1.3MID大鼠動(dòng)物模型的建立按陳俊拋1，2報(bào)道的方法制作MID大鼠動(dòng)物模型。自頸外動(dòng)脈緩慢注入80 m200 m的自體血栓干粉，拔管并結(jié)扎頸外動(dòng)脈起始部，確認(rèn)無(wú)出血后縫合手術(shù)切口。術(shù)后觀察大鼠呼吸、肢體活動(dòng)等情況。假手術(shù)組除注入等量的生理鹽水外，其余處理均同模型組。1.4實(shí)驗(yàn)動(dòng)物分組將SD大鼠隨機(jī)分為四組：假手術(shù)組、模型組、西藥組、銀杏葉組。每組10只，采用灌胃給藥：中藥組按150 mg/kg給藥，西藥組按0.3 mg/kg給藥，假手術(shù)組與模型組以相同體積的生理鹽水灌胃，每組大鼠單次連續(xù)給藥6 d，第7天測(cè)試游水迷宮及電刺激試驗(yàn)，測(cè)試期間停止灌藥。實(shí)驗(yàn)動(dòng)物飼養(yǎng)于19 25 的環(huán)境中，自由飲水，自然光照。1.5記憶行為測(cè)試游水迷宮試驗(yàn)：以癡呆大鼠自水迷宮起點(diǎn)游至終點(diǎn)安全臺(tái)所要時(shí)間，作為衡量學(xué)習(xí)獲得能力的指標(biāo)。傷害性電刺激試驗(yàn)：采用自制的3被動(dòng)回避能力測(cè)試的兩隔離室電擊