Kinetin-riboside-N6-Furfuryladenosine-DataSheet-生命科學(xué)試劑-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEKinetin ribosideCat. No.: HY-101055CAS No.: 4338-47-0Synonyms: N6-Furfuryladenosine分式: CHNO分量: 347.33作靶點(diǎn): Apoptosis作通路: Apoptosis儲存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實(shí)驗(yàn) DMSO : 29 mg/mL (83

2、.49 mM)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制備儲備液1 mM 2.8791 mL 14.3955 mL 28.7911 mL5 mM 0.5758 mL 2.8791 mL 5.7582 mL10 mM 0.2879 mL 1.4396 mL 2.8791 mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液，并請注意儲備液的保存式和期限。體內(nèi)實(shí)驗(yàn)請根據(jù)您的實(shí)驗(yàn)動物和給藥式選擇適當(dāng)?shù)娜芙獍?，配制前請先配制澄清的儲備液，再依次添加助溶?為保證實(shí)驗(yàn)結(jié)果的可

3、靠性，體內(nèi)實(shí)驗(yàn)的作液，建議您現(xiàn)現(xiàn)配，當(dāng)天使；澄清的儲備液可以根據(jù)儲存條件，適當(dāng)保存；以下溶劑前的百分 指該溶劑在您配制終溶液中的體積占)：1. 請依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (7.20 mM); Clear solution2. 請依序添加每種溶劑： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (7.20 mM); Clear solution1/2 Master of Small Molecules 您邊

4、的抑制劑師www.MedChemE3. 請依序添加每種溶劑： 10% DMSO 90% corn oilSolubility: 2.5 mg/mL (7.20 mM); Clear solutionBIOLOGICAL ACTIVITY物活性 Kinetin riboside是細(xì)胞分裂素類似物，可誘導(dǎo)癌癥細(xì)胞死亡。抑制HCT-15細(xì)胞增殖的IC50值為2.5 M。IC50 & Target IC50: 2.5 M (HCT-15 cells) 1體外研究 Kinetin riboside displays antiproliferative and apoptogenic activity a

5、gainst various human cancer cell lines.Kinetin riboside is able to inhibit the proliferation in HCT-15 human colon cancer cells in a dose-dependentmanner (IC50=2.5 M) 1. Kinetin riboside induces apoptosis in HeLa and mouse melanoma B16F-10 cells.Kinetin riboside disrupts the mitochondrial membrane p

6、otential and induces the release of cytochrome c andactivation of caspase-3. Bad are up-regulated while Bcl-2 is down-regulated under kinetin riboside exposure2.體內(nèi)研究 Kinetin riboside significantly suppresses tumor growth. The most effective anti-melanoma response is elicitedat 40 mg/kg 2.PROTOCOLCel

7、l Assay 2 HeLa and mouse melanoma B16F-10 cells are treated with 5, 10, 20 M kinetin riboside for 48 h. 15 L ofMTT solution (5 mg/mL) is added to each well and cells are maintained for 4 h at 37C. Hundred microlitresof solubilizing solution is then added. After an overnight incubation at room temper

8、ature, absorbance at 490nm is measured 2.MCE has not independently confirmed the accuracy of these methods. They are for reference only.Animal Mice: Male C57BL/6 mice are injected B16 F-10 cells. After 5 days for tumor growth, kinetin riboside (10, 20,Administration 2 40 mg/kg) is injected to tumor

9、mass directly. Drug injection is performed once a 3 days for three times. Afterthird injection of drug, mice are kept for 3 days with no injection and tumor mass is removed from eachmouse and weighed 2.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REF

10、ERENCES1. Rajabi M, et al. Antiproliferative activity of kinetin riboside on HCT-15 colon cancer cell line. Nucleosides Nucleotides Nucleic Acids.2012;31(6):474-81.2. Choi BH, et al. Kinetin riboside preferentially induces apoptosis by modulating Bcl-2 family proteins and caspase-3 in cancer cells.Cancer Lett. 2008 Mar 8;261(1):37-45.McePdfHeightCaution: Product has not been fully va