捕獲和釋放活性細胞的方法

Captureandreleaselivecells匯報人：于倩茹LIVECELLSCirculatingTumorsCells(CTCs)TLymphocytesCirculatingTumorsCells(CTCs)Circulatingtumorcells(CTCs)arecancercellsthatbreakawayfromeitheraprimarytumororametastaticsiteandcirculateintheperipheralbloodasthecellularoriginofmetastasis.Notion:Why?DetectingandanalyzingCTCsProvideinsightfulinformationinassessingthediseasestatusCTCsprovideconvenientaccesstoalldiseasesites現代技術中捕獲和釋放CTCs的主要方法根據分離原理基于物理性質分離基于親和識別分離基于親和識別分離利用癌細胞與正常血細胞的差別密度變形性粘附性尺寸基于物理性質分離核酸適配體抗體

NanostructureEmbeddedMicrochipsforDetection,Isolation,and

CharacterizationofCirculatingTumorCells基于親和識別-抗體-實例Workingmechsnism: Whenthetwofabricstrips

ofaVelcrofastenerarepressedtogether,tanglingbetweenthe

hairysurfacesontwostripsleadstoenhancedbinding.The

proof-of-conceptdemonstrationoftheNanoVelcrocell-affinity

assay

liesintheuseofaSiNS,whichallowsforVelcro-likeinteractions

betweentheSiNSandnanoscalecell-surface

components.Here,anti-EpCAMwasgraftedontotheSiNSas

thecaptureagent,conferringspecificitytosuchacell-affinity

assayforrecognizingCTCs.Anti-EpCAMisgraftedontoaSiNStoconferspecificity

forrecognizingCTCs.Theanti-EpCAMgraftedSiNS

wasfabricatedthroughthreecontinuoussteps:(i)

introductionofdenselypackedsiliconnanowiresontoasilicon

wafer(ii)silanetreatmentandcovalentconjugationofstreptavidinontotheSiNS(iii)graftingofbiotinylated

anti-EpCAMontothestreptavidin-coatedSiNS.(a)Ananti-EpCAM-coatedSiNSwasemployedtoachievesignificantlyenhancedcaptureofCTCsincontrastto(b)ananti-EpCAM-coatedflatsiliconsubstrate。TLymphocytes

Tlymphocyteshavefundamentalfunctionsinimmuneresponsestomanydiseases,suchaspathogenicinfections,malignancies,andautoimmunediseases.DetectionsoftargetedTlymphocytesprovideimportantinformationinclinicaldiagnosis

.

TocaptureandmodifyTlymphocytestomakethemgetmorepowerfulPhotodegradableHydrogelsforCapture,Detection,andReleaseofCD4T-cellsandCD8T-cells實例：AntibodyprintingonthephotogellayerCellcaptureExposuretoUVlightCellreleaseExperimentProcedurePhotodegradableHydrogelsWeusedPEGhydrogel

asacoatingforprintingantibodyarraysandcapturingT-cellsfromaperipheralbloodmononuclearcell(PBMC)suspensionFormationofspotswithadiameterofapproximately150μmCellCaptureTheunderlyin