常用凝膠參數(shù)

1、常用凝膠G技術(shù)參數(shù)1.葡聚糖凝膠內(nèi)某些技術(shù)數(shù)據(jù)種類(lèi)干顆粒直徑小分子量分級(jí)范圍床體積毫升/克干分子篩得水值溶脹最少平衡時(shí)間h柱頭壓力kPa2.5cm直徑柱肽及球形蛋白質(zhì)葡聚糖線(xiàn)性分子室溫沸水浴SephadexG-1040120700700231.00.131SephadexG-1040120150015002.53.51.53.531SephadexG-25粗級(jí)100300(=5100目)中級(jí)50150(-100200目)1,0005,0001005,000461.50.262細(xì)級(jí)20800(弋200400目)超細(xì)1040SephadexG-50粗級(jí)100200中級(jí)501501,500500細(xì)級(jí)

2、208030,00010,0009115.00.362超細(xì)1040SephadexG-75401203,0001,000超細(xì)104070,000950,00012157.50.52433.9215.86SephadexG-100401204,0001,000超細(xì)10401,500,00050,000152010.01.04852.359.41SephadexG-150401205,0001,0002030超細(xì)1040400,000150,000182215.01.57250.883.53SephadexG-200401205000100030401040800,000200,000202520

3、.02.07250.391.572.聚丙烯酰胺凝膠CD技術(shù)數(shù)據(jù)型號(hào)排阻內(nèi)下限分子量分級(jí)別離范圍分子量膨脹后內(nèi)床體積ml/g干凝膠膨脹所需最少時(shí)間室溫,hBio-gel-P-21,6002002,0003.824Bio-gel-P-43,6005004,0005.824Bio-gel-P-64,6001,0005,0008.824Bio-gel-P-1010,0005,00017,00012.424Bio-gel-P-3030,00020,00050,00014.91012Bio-gel-P-6060,00030,00070,00019.01012Bio-gel-P-100100,00040,0

4、00100,00019.024Bio-gel-P-150150,00050,000150,00024.024Bio-gel-P-200200,00080,000200,00034.048Bio-gel-P-300300,000100400,00040.0483.瓊脂糖凝膠內(nèi)技術(shù)數(shù)據(jù)型號(hào)瓊脂糖含量%W/vy排阻內(nèi)下限分子量分級(jí)別離內(nèi)范圍分子量生產(chǎn)廠(chǎng)家Sepharose4B40.3X1063X106PharmaciaSepharose2B22X10625X106Sagavac10102.5X1051X1042.5X105SeravacSagavac887X1052.5X1047X105Sagava

5、c662X1065X10-2X106Sagavac4415X1062X10515X106Sagavac22150X1065X10515X107Bio-gelA-0.5M100.5X1061X104-0.5X106Bio-gelA-1.5M81.5X106密子第位1端CUALeuCCAProCAAGlnCGAArgACUGLeuCCGProCAGGlnCGCArgGAUUIleACUThrAAUAsnAGUSerAUCIleACCThrAACAsnAGCSerqAAUAIleACAThrAAALysAGAArgAAUGMetACGThrAAGLysAGCArgGUUValGCUAlaGAUAsp

6、GGUGly口GUCValGCCAlaGACAspGGCGlyGGUAValGCAAlaGAAGluGGAGlyAGUGValGCGAlaGAGGluGGGGly目九、常用酸堿技術(shù)參數(shù)1.常見(jiàn)內(nèi)市售酸堿內(nèi)濃度溶質(zhì)分子式分子量mol/Lg/L重量(%比重配制mol/L溶液內(nèi)參加量(ml/L)冰乙酸CHCOOH60.0517.40104599.51.05057.5乙酸60.056.27376361.04559.5甲酸HCOOH46.0223.40080901.200z工2.7鹽酸HCl36.5011.604124361.18036.22.90105101.050344.8硝酸HNO63.0215.

7、991008711.42062.514.90938671.40067.113.30f337611.37075.2高氯酸HclOa100.5011.651172701.67085.89.20923601.540108.7磷酸HPO80.0018.101445851.70055.2硫酸H2PQ98.1018.00776961.84055.6多氧化錢(qián)NHOH35.0014.80:?51280.89857.6多氧化鉀KOH56.1013.507節(jié)7501.52074.11.9410910II1.090515.5多氧化鈉NaOH40.0019.10,63501.53052.42.75111101.110363.42.各種濃度CD酸堿貯存液CD近似pH值溶質(zhì)1N30.1Na0.01Na0.001Na乙酸0.402.903.403.90鹽酸0.101.072.023.01硫酸0.