參考文稿說明report

1、Penetration Modeling of Shaved Skin StudyResearch Progress Guoqiang JIANG , Tsinghua University In vitro penetration studySkin： Porcine abdominal skin （Bama miniature pig）Donor: Gel(Nicotinamide) / Caffeine aqueous solution (5 g/L) / VitaminEOil solutionReceiver: Water (pH=7.3), 6 mLTemperature: 32

2、No treatment (Control)Shaving without foamShaving with foamTaping after ShavingIn vitro penetration of Nicotinamide Accumulative percentage penetration of Nicotinamide (Gel formation)Nicotinamide entrapped within skin In vitro penetration of Nicotinamide Mass on the Tapes: Shaving 1.8 mg/tape Shavin

3、g + Foam 1.8 mg/tapeIn vitro penetration of Nicotinamide 2st tape5ed tape Shaving Shaving+FoamAccumulative penetration percentage of Nicotinamide (Gel formation，After 5 tapes) Nicotinamide entrapped within skinIn vitro penetration of Nicotinamide In vitro penetration of VitaminEAccumulative percenta

4、ge penetration of Vitamin E (Oil solution, 0.5mg/mL)In vitro penetration study of VitaminEVitamin E entrapped within skin In vitro penetration of CaffeineAccumulative percentage penetration of caffeineIn vitro penetration of CaffeineAccumulative penetration percentage of caffeine (Donor: Solution, 5

5、 g/L) Mass on Tapes: Shaving 3mg Shaving +Foam 2.6 mg In vitro penetration of CaffeineConfocal Raman Spectroscopy Raman spectrum of skin (No Treatment+ caffeine)Confocal Raman SpectroscopyRaman spectrum of skin (Shaved + caffeine)Confocal Raman SpectroscopyRaman spectrum of skin (Shaved + foam + caf

6、feine)Confocal Raman SpectroscopyNo TreatmentShaved without foamShaved with foamPathway study by CLSMFluorescein isothiocyanate isomer I（FITC）CalceinZeiss LSM 710HeNe 綠色激光器 543nmSkin Porcine abdominal skin （Bama miniature pig）In vitro penetration of FITC in 1 hourIn vitro penetration of Calcein in 1 hourPathway study by CLSMdepthShaved without foamNo TreatmentShaved with foamPenetration of FITCPenetration of CalceinShaved without foamShaved with foamNo TreatmentdepthDiscussionsThe indirect eviden