如何用NCBI和uniprot數(shù)據(jù)庫查找目的蛋白的氨基酸序列或目的基因的堿基序列(mRNA序列)

運用NCBI數(shù)據(jù)庫查找目得蛋白序列或目得堿基序列PruteinHomologyFubChamCEIIMssonlQrganizationINCeormanuscrptsTransferNCBIdatatoyourFindhelpdocumerts.atendaptabasegclassarnatchatutonalUseNCBIAPlsandcodelibrariestobuldapolicationgmorg._ChromosomaX. CL5.HU-3.ISFK-1MAPKAPK1B,MRX19,RSK.RSK2,96K-alzha3,pRSK3、在搜索結果中查找目得DescriptionhumanAliases欄會列出目得蛋白得各種別名。_CLS,HU-3,ISPK-1,下 SeeidenticalproteinsandtheirannotatedlocationsforNP004577.1NCBIisphasingoutsequenceGlnumbersinSeptemberPleaseuseLOCUSNM0045867723bpmRJAlinsarPRID3-LAR-2016DEFINITIOIHcmosapieneribosonalproteinS6kinaseA3(RPS6EA3),mRUA.____RefSeq.EukaryotaMetazoaChordataCraniataVertebcataEuteleostcmMammalia,Eutheria,Euarchontoglires;PrimatesHaplorrhiniCatarrhini,Hominidae;Homo.中善第2中方法Automaticallyannotatedandnotreviewed.UniProtKB-UniProtKB-?Pathology&Biotoch又IFamly8DamainsSernethreonineproteinkinasesurvival,anddifferentiationbymodulatingmTORsignalingandrepressingpro-apoptoticfunctionofBADphosphorylationofCREB1andhistoneH3at'Ser-10',whichresultsinthesubsequenttranscriptionalacmitogenicstimulationEGFandPMderivedsignal,actsindirectlyonthetranscriptionregulationofseveralgenesbyphosphorylatingGSK38responsetoserumorEGFviaanmTORindependentmechanismandpromtoinsulin,phosphorylatesElF4B,enhancingEIF4BaffinityfortheEIF3complexandstimulatingcap-deppathwaybydirectlyphosphorylatingTSC2at'Ser-1798',whichpotentlyinhibitsTSC2abilitytosuppreswhichregulatesmTORC1activityandmaypromoterapamycin-sensitivesignalingindependentlyofthethepro-apoptoticproteinsBADandDAPKIandsuppressingtheirpro-apoptoticfunction.Promotestheresponsetothehepatotoxincarbontetrachloride(CCl4).Isinvolvedincellcycleregulationbyphosphoassociationwith14-3-3proteinsandpreventsitstranslocationtothenucleusandinhibitionofGlprogindhrormarmninncwhncicandinmvelnm=rollesrheseoffertnrnFECED3-moristaddtranefnrahinncintRpsksMapkapkMusmuscuusMoAps6ks-151,Rsk2apskaMapkapkicMusmuscul Mitogen-activatesdprotsinkinasa1Mitogen-activatedproteinkinace360Mitogen-activatedproteinkinase1MapkErkMapkPrkmlMsmuscuusMou4.點擊頁面頁面左側得Sequence欄，并找到Sequencedatabases,其下面表格中有RefSeg,其中包含NP及NM、SequencedatabasesNaneSelectthelinkdestinations:ieentaonCA396871AB102662mRNATasanBA811311擔索墓制5、此處以查找mRNA為例，點擊NM,頁面會跳轉至NCBI得查找頁面，如下：1 P9UKSKZPPUKSKKSKKSKZSbKalphap90-RSK2;pp9ORSK2;RSK;RSK2;S6K-alpha3cccstccgaccaaactgaagaagtcagtatcaaagaaattgcaatcacacatcatgtaaaggaaggacatzaaaaggcagatccttcccagtttgaacttttaaaagtattaggecagggatcatttggaaaggttttcttagttaaaaaaatctcagectctgatgctaggcagctttateccatgaaggggaagttgtggattttctcgaccgagttcgtcaagttecagrggaggaggpacaaaaatattatectttatttgtttacegaacgtgattcaaactgaaacgcttatccaaagasgtratattcacagaagaaxatgtctrgctsaacttagaccatctacatagcctgggaataatttatagagacttaaaaccagaaaatatactttttactgcaaaaactcccaaagattcacctggcattccac