微衛(wèi)星DNA標(biāo)記與涼山半細(xì)毛羊羊毛性狀關(guān)系的研究

1、微衛(wèi)星DNA標(biāo)記與涼山半細(xì)毛羊羊毛性狀關(guān)系的研究動(dòng)物遺傳育種與繁殖：胡容 指導(dǎo)教師：吳登俊教授摘 要本試驗(yàn)選用綿羊基因組中第3號(hào)染色體上的13個(gè)微衛(wèi)星標(biāo)記和X染色體上的2個(gè)微衛(wèi)星標(biāo)記，結(jié)合PCR和復(fù)合電泳銀染技術(shù)對(duì)涼山半細(xì)毛羊核心育種群6個(gè)父系組成的129只個(gè)體的7個(gè)羊毛表型性狀進(jìn)行標(biāo)記表型相關(guān)研究。7個(gè)表型性狀為：斷奶毛長(zhǎng)（SL1），育成毛長(zhǎng)（SL2），成年毛長(zhǎng)（SL3），育成剪毛量（WY1），成年剪毛量（WY2），育成毛纖維直徑（WFD1），成年毛纖維直徑（WFD2）。對(duì)7個(gè)羊毛性狀進(jìn)行表型相關(guān)分析，育成毛長(zhǎng)與成年毛長(zhǎng)之間呈極顯著正相關(guān)（P<0.01）；育成剪毛量與成年剪毛量之間呈極

2、顯著正相關(guān)（P<0.01）；育成毛細(xì)度與斷奶毛長(zhǎng)呈顯著負(fù)相關(guān)（0.01<P<0.05），還與成年毛細(xì)度呈顯著正相關(guān)（0.01<P<0.05）；成年毛細(xì)度與育成剪毛量呈極顯著負(fù)相關(guān)（P<0.01）。所選微衛(wèi)星標(biāo)記在涼山半細(xì)毛羊群體中平均檢測(cè)到7.470個(gè)（511個(gè)）等位基因，平均多態(tài)信息含量為0.754（0.5520.859），群體雜合度平均值為0.705（0.5410.817）。15個(gè)標(biāo)記均呈現(xiàn)高度或中度多態(tài)性，為該群體分子水平上的研究提供了可靠的依據(jù)。 通過(guò)微衛(wèi)星標(biāo)記與表型性狀的相關(guān)分析，結(jié)果發(fā)現(xiàn)9個(gè)微衛(wèi)星標(biāo)記對(duì)部分羊毛性狀存在顯著或極顯著影響。其中標(biāo)記

3、BMS0460、BM3501、BL0004對(duì)斷奶毛長(zhǎng)影響顯著（P<0.05），AE25對(duì)斷奶毛長(zhǎng)影響極顯著（P<0.01）；ILSTS22對(duì)育成毛長(zhǎng)影響顯著（P<0.05）；Oarcp43、MILVET09對(duì)育成剪毛量影響分別為極顯著（P<0.01）和顯著（P<0.05）；RM150、ILSTS22、ILSTS0049對(duì)成年剪毛量影響顯著（P<0.05），MILVET09對(duì)成年剪毛量影響極顯著（P<0.01）；Oarcp43對(duì)成年毛細(xì)度影響顯著（P<0.05），AE25對(duì)成年毛細(xì)度影響極顯著（P<0.01）。通過(guò)對(duì)與表型顯著相關(guān)的標(biāo)記進(jìn)行不

4、同基因型間的多重比較，結(jié)果發(fā)現(xiàn)在標(biāo)記BMS0460中，等位基因G對(duì)斷奶毛長(zhǎng)起增效作用，基因型GG、DG、BB為優(yōu)勢(shì)基因型。在標(biāo)記Oarcp43中，基因型BD是育成剪毛量的優(yōu)勢(shì)基因型，基因型DD是成年毛細(xì)度的優(yōu)勢(shì)基因型。在標(biāo)記BM3501中，II是影響斷奶毛長(zhǎng)的優(yōu)勢(shì)基因型，等位基因I為優(yōu)勢(shì)基因。在標(biāo)記RM150中，等位基因F、C對(duì)成年剪毛量分別起增效和減效作用，基因型AF為優(yōu)勢(shì)基因型。在標(biāo)記BL0004中，基因型CE 、BC、EE為斷奶毛長(zhǎng)的優(yōu)勢(shì)基因型。在標(biāo)記ILSTS22中，基因型DD、EF、DE是育成毛長(zhǎng)的優(yōu)勢(shì)基因型，等位基因D起增效作用；基因型FF是成年剪毛量的優(yōu)勢(shì)基因型，等位基因F、E分

5、別對(duì)成年剪毛量起增效作用和減效作用。在標(biāo)記MILVET09中，基因型BB、AC、BC同時(shí)為育成剪毛量和成年剪毛量?jī)蓚€(gè)性狀的優(yōu)勢(shì)基因型。在標(biāo)記AE25中，基因型EG為斷奶毛長(zhǎng)的優(yōu)勢(shì)基因型，基因型EF為成年毛細(xì)度的優(yōu)勢(shì)基因型。在標(biāo)記ILSTS0049中，基因型AG是成年剪毛量性狀的優(yōu)勢(shì)基因型。關(guān)鍵詞：微衛(wèi)星標(biāo)記；涼山半細(xì)毛羊；羊毛性狀；相關(guān)分析The Relation of Microsatellite DNA Markers with Wool Traits in Liangshan Semi-wool SheepHu Rong (Animal genetics,breeding and rep

6、roduction)Directed by Wu Deng-junAbstractA marker-phenotype correlative analysis of 7 phenotype wool traits of 129 individuals of 6 sires in a Liangshan Semi-wool Sheep nucleus breeding population was made by adopting thirteen microsatellite markers on chromosome 3 and two microsatellite markers on

7、chromosome X in sheep genome, binding polymerase chain reaction (PCR) and multiplex gel electrophoresis followed by silver staining. The 7 phenotype traits were:staple length,staple length at 18 months,staple length at 30 months,wool yield at 18 months,wool yield at 30 months,wool fibre diameter at

8、18 months,wool fibre diameter at 30 months. The conclusion of phenotype correlative analysis of 7 wool traits is that:staple length at 18 months is extreme significantly correlated with staple length at 30 months(p<0.01); wool yield at 18 months is extreme significantly correlated with wool yield

9、 at 30 months (p<0.01);wool fibre diameter at 18 months is significantly correlated negatively with staple length（0.01<P<0.05）, is significantly correlated with wool fibre diameter at 30 months（0.01<P<0.05）; wool fibre diameter at 30 months is extreme significantly correlated negative

10、ly with wool yield at 18 months（P<0.01）.The results of this experiment are as following:the average number of alleles for 15 microsatellite markers is 8 (from 5 to 11); the average polymorphic information is 0.754（from 0.552 to 0.859）;the average heterozygosity is 0.705(from 0.541 to 0.817).The p

11、olymorphic information of all the microsatellite markers are at a high or middle level,so can supply a credible information for this study.The microsatellite markers-phenotype correlative analysis uncovered that 9 microsatellite markers had a significant or extreme significant impact on part of wool

12、 traits.They are as following:the marker BMS0460,BM3501 and BL0004 have a significant impact on Staple length (p<0.05),and the marker AE25 is extreme significantly(p<0.01);the marker ILSTS22 has a significant impact on staple length at 18 months（P<0.05）;the markerOarcp43, MILVET09 have a ex

13、treme significant(p<0.01) and significant（P<0.05）impact on wool yield at 18 months;the marker RM150, ILSTS22, ILSTS0049 have a significant impact on wool yield at 30 months（P<0.05）,and the marker MILVET09 is extreme significantly(p<0.01);the marker Oarcp43 have a significant impact on wo

14、ol fibre diameter at 30 months（P<0.05）,and the marker AE25 is extreme significantly(p<0.01).A multiple comparison was made between genotypes and the markers which are significantly correlated with the phenotype trait.The results are as following:in the marker BMS0460,allele G has additive effe

15、ct to staple length,and genotypes GG,DG,BB are the dominant genotype; in the marker Oarcp43,genotype BD is the dominant genotypes for wool yield at 18 months, genotype DD is the dominant genotypes for wool fibre diameter at 30 months; in the marker BM3501,genotype II is the dominant genotypes for st

16、aple length,and allele I is the dominant allele;In the marker RM150,allele F has additive effect to wool yield at 30 months,and allele C is depletive, and genotype AF is the dominant genotypes;in the marker BL0004, genotypes CE,BC and EE are the dominant genotypes for staple length;in the marker ILS

17、TS22,genotypes DD,EF and DE are the dominant genotypes for staple length at 18 months,allele D is the dominant allele. Genotype FF is the dominant genotype for wool yield at 30 months, allele F has additive effect to wool yield at 30 months,and allele E is depletive;in the marker MILVET09, genotypes BB,AC,BC are the dominant genotypes for wool yield at 18 months and wool yield at 30 months;in the marker AE25, genotype EG is the dominant gen