鹽酸小檗堿水合物作用機制 - Medchemexpress - MCE中國

1、Product Data SheetBerberine chloride hydrateCat. No.: HY-17577CAS No.: 68030-18-2分式: CHClNO分量: 389.83作靶點: Topoisomerase; Autophagy; Bacterial; Reactive Oxygen Species作通路: Cell Cycle/DNA Damage; Autophagy; Anti-infection; Immunology/Inflammation;Metabolic Enzyme/Protease; NF-B儲存式: Powder -20C 3 years

2、4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實驗 DMSO : 3.9 mg/mL (10.00 mM)H2O : 1.25 mg/mL (3.21 mM; Need ultrasonic)* means soluble, but saturation unknown.SolventMass1 mg 5 mg 10 mgConcentration制備儲備液1 mM 2.5652 mL 12.8261 mL 25.6522 mL5 mM 0.5130 mL 2.5652 mL 5.1304 mL10 mM 0.2565 mL 1.28

3、26 mL 2.5652 mL請根據(jù)產品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；旦配成溶液，請分裝保存，避免反復凍融造成的產品失效。儲備液的保存式和期限：-80C, 6 months; -20C, 1 month。-80C 儲存時，請在 6 個內使，-20C 儲存時，請在 1 個內使。BIOLOGICAL ACTIVITY物活性 Berberine chloride hydrate (Natural Yellow 18 chloride hydrate) 從中草藥 連中分離出來的種物堿，常作抗 素。Berberine chloride hydrate 誘導活性氧 (ROS) 成并抑制 D

4、NA 拓撲異構酶 (topoisomerase)?？鼓[瘤特性1。IC & Target ROS1DNA topoisomerase1體外研究Berberine chloride hydrate ( Natural Yellow 18 chloride hydrate; 1.25-160 M; 72 hours) has potential inhibitoryeffects on the proliferation of four colorectal carcinoma cell lines LoVo, HCT116, SW480, and HT-291.Berberine chloride

5、 hydrate (1.25-160 M; 24-72 hours) induces a time- and dose-dependent inhibition of LoVo cellPage 1 of 2 www.MedChemEgrowth1.LoVo cells are exposure to Berberine chloride hydrate (10-80 M) for 24 h. Cell cycle analysis of 40 M Berberine-treated LoVo cells by flow cytometry shows accumulation of cell

6、s in the G2/M phase1.Berberine chloride hydrate (10-80 M) suppresses cyclin B1, cdc2 and cdc25c protein expression after 24 h, especiallyat the dose of 80.0 M1.Cell Proliferation Assay1Cell Line: Four colorectal carcinoma cell lines LoVo, HCT116, SW480, and HT-29Concentration: 1.25, 2.5, 5, 10, 20,

7、40, 80, and 160 MIncubation Time: 72 hoursResult: Inhibited the proliferation of four cell lines. The IC50 ranged from 40.84.1 M (LoVo)to 98.62.9 M (HCT116).Cell Proliferation Assay1Cell Line: Colorectal carcinoma cell lines LoVoConcentration: 1.25, 2.5, 5, 10, 20, 40, 80, and 160 MIncubation Time:

8、24, 48, 72 hoursResult: Induced a time- and dose-dependent inhibition of cell growth. By 72 h, 160.0 Minduced 71.11.9 % growth inhibitions in LoVo cells.Cell Cycle Analysis1Cell Line: LoVo cellsConcentration: 0, 10, 20, 40, or 80 MIncubation Time: 24 hoursResult: Exposure to 40.0 M induced G2/M-phas

9、e cell cycle arrest, an increase in the G2/M-phase population and a progressive decline in the G1 population.Western Blot Analysis1Cell Line: LoVo cellsConcentration: 10, 20, 40, or 80 MIncubation Time: 24 hoursResult: Suppressed cyclin B1, cdc2 and cdc25c protein expression.體內研究Berberine chloride h

10、ydrate (Natural Yellow 18 chloride hydrate; 10, 30, or 50 mg/kg/day; gastrointestinal gavage; for10 consecutive days) inhibits the growth of human colorectal adenocarcinoma in vivo. Berberine chloride hydrate atdoses of 30 and 50 mg/kg/day taken by gastrointestinal gavage shows inhibitory rates of 3

11、3.1% and 45.3% on thehuman colorectal adenocarcinoma xenograft growth in nude mice1.Animal Model: 5-week-old BALB/c nu/nu mice with human colorectal adenocarcinoma LoVoxenografts1Page 2 of 3 www.MedChemEDosage: 10, 30, or 50 mg/kg/dayAdministration: Gastrointestinal gavage; for 10 consecutive daysRe

12、sult: Showed inhibitory rates of 33.1 % and 45.3 % at doses of 30 and 50 mg/kg/day.戶使本產品發(fā)表的科研獻 Exp Cell Res. 2020 May 3:112054.See more customer validations on HYPERLINK www.MedChemE www.MedChemEREFERENCES1. Cai Y, et al. Berberine inhibits the growth of human colorectal adenocarcinoma in vitro and in vivo. J Nat Med. 2014 Jan;68(1):53-62.McePdfHeightCaution: Product has not bee