Heparan-Sulfate - FGFR - 生命科學(xué)試劑 - MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEHeparan SulfateCat. No.: HY-101916CAS No.: 9050-30-0分式: CHNOS (monomer)作靶點: FGFR; Wnt; Endogenous Metabolite作通路: Protein Tyrosine Kinase/RTK; Stem Cell/Wnt; MetabolicEnzyme/Protease儲存式: Powder -20C 3 years4C 2 yearsIn solvent -8

2、0C 6 months-20C 1 month溶解性數(shù)據(jù)體外實驗 H2O : 47.1 mg/mL (Need ultrasonic and warming)請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液，并請注意儲備液的保存式和期限。BIOLOGICAL ACTIVITY物活性 Heparan sulfate種復(fù)雜的線性多糖。它作為硫酸酰肝素蛋聚糖的部分出現(xiàn)，在細(xì)胞表和細(xì)胞外基質(zhì)中表達(dá)豐富。IC50 & Target Human Endogenous Metabolite體外研究Heparan sulfate is a linear polysaccharide and belon

3、gs to the family of glycosaminoglycans. Heparan sulfateis composed of glucuronic acid (GlcA) and iduronic acid (IdoA) residues as well as N-acetyl glucosamines(GlcNAc) with various sulfation modifications, and is typically 50-200 disaccharides in length. Heparan sulfateinteracts with numerous protei

4、ns, including growth factors, morphogens, and adhesion molecules, andthereby regulates important developmental processes in invertebrates and vertebrates. Heparan sulfatechains regulate developmental signaling by acting as co-factors through a variety of mechanisms that includebut are not limited to

5、 maintenance of morphogen gradients and co-receptor functions 1. Heparan sulfateproteoglycans can act as receptors for proteases and protease inhibitors regulating their spatial distributionand activity. Membrane Heparan sulfate proteoglycans act as coreceptors for various tyrosine kinase-typegrowth

6、 factor receptors, lowering their activation threshold or changing the duration of signaling reactions 2.Heparan sulfate influences the binding affinity of intestinal epithelium cells to Wnt, thereby promoting1/2 Master of Small Molecules 您邊的抑制劑師www.MedChemEactivation of canonical Wnt signaling and

7、facilitating regeneration of small intestinal crypts after epithelialinjury 3.體內(nèi)研究 Digestion of heparan sulfate impairs context discrimination in a fear conditioning paradigm and oscillatorynetwork activity in the low theta band after fear conditioning. Thus, heparan sulfate maintains neuronalexcita

8、bility and, as a consequence, support synaptic plasticity and learning 4. FGF-2/FGFR system isinvolved in the regulation of astrocytic reactivity and/or proliferation in the brain and its action is potentiatedby heparan sulfate 5.PROTOCOLAnimal Rats: Sprague-Dawley male rats 3-4 months old receive a

9、spiration of a small segment of the motor-sensoryAdministration 5 cortex in both hemispheres. Groups of rats (n=5) receive a piece of Gelfoam embedded with humanrecombinant FGF-2, heparan sulfate (10 kg/mL; sodium salt, bovine kidney) dissolved in PBS, or both, in theright hemisphere. The wound cavi

10、ty of the left hemisphere receives a piece of Gelfoam embedded with saline5.MCE has not independently confirmed the accuracy of these methods. They are for reference only.戶使本產(chǎn)品發(fā)表的科研獻(xiàn) Carbohydr Polym. 2019 Jul 1;215:73-81. Carbohyd Polym. 2019 Mar 1;207:191-199.See more customer validations on HYPERL

11、INK / www.MedChemEREFERENCES1. Kraushaar DC, et al. Heparan sulfate: a key regulator of embryonic stem cell fate. Biol Chem. 2013 Jun;394(6):741-51.2. Sarrazin S, et al. Heparan sulfate proteoglycans. Cold Spring Harb Perspect Biol. 2011 Jul 1;3(7). pii: a004952.3. Yamamoto S, et al. Heparan sulfate

12、 on intestinal epithelial cells plays a critical role in intestinal crypt homeostasis via Wnt/-cateninsignaling. Am J Physiol Gastrointest Liver Physiol. 2013 Aug 1;305(3):G241-9.4. Minge D, et al. Heparan Sulfates Support Pyramidal Cell Excitability, Synaptic Plasticity, and Context Discrimination. Cereb Cortex. 2017Feb 1;27(2):903-918.5. Gmez-Pinilla F, et al. Regulation of astrocyte proliferation by FGF-2 and heparan sulfate in vivo. J Neurosci. 1995 Mar;15(3 Pt 1):2021-9.McePdfHeightC